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Invitrogen
LIMP2 Recombinant Rabbit Monoclonal Antibody (22H6L14)
FIGURE: 1 / 7
LIMP2 Antibody (703037) in ICC/IF
Knockdown of SCARB2 was achieved by transfecting A549 cells with SCARB2 siRNA (Silencer® select Product # S2652, S2653). Immunofluorescence analysis was performed on untransfected A549 cells (panel a, d), transfected with non-specific scrambled siRNA (panels e, h) and transfected with SCARB2 specific siRNA (panel i, l). Cells were fixed, permeabilized, and labelled with LIMP2 Recombinant Rabbit Monoclonal Antibody (22H6L14) (Product # 703037, 1:100 dilution) followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More
Product Details
703037
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
22H6L14
Immunogen
Protein corresponding to human SCARB2 [aa35-aa430]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734813
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. Although the role of SR-BII is not completely clear, research suggests that it may be a functional HDL receptor. In addition, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 85 kDa lysosomal membrane sialoglycoprotein; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; CD 36; CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II); CD36 antigen-like 2; CD36 molecule; LGP85; LIMP II; Lysosome membrane protein 2; Lysosome membrane protein II; Scavenger receptor class B member 2; scavenger receptor class B, member 2; sCD 36; sCD36; soluble CD 36; soluble CD36; SR-B2
Gene Aliases: AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2; LIMP2; LIMPII; SCARB2; SR-BII
UniProt ID: (Human) Q14108
Entrez Gene ID: (Human) 950
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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