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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • LIN28A Antibodies

          Proteintech

          LIN28 Polyclonal Antibody

          59 References
          View all (27) LIN28A antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite LIN28 Polyclonal Antibody

          • Antibody Testing Data (9)
          LIN28 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          LIN28 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          LIN28 Antibody (11724-1-AP) in ICC/IF

          Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LIN28 Antibody in Immunocytochemistry (ICC/IF)
          LIN28 Antibody in Immunocytochemistry (ICC/IF)
          LIN28 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LIN28 Antibody in Western Blot (WB)
          LIN28 Antibody in Western Blot (WB)
          LIN28 Antibody in Western Blot (WB)
          LIN28 Antibody in Western Blot (WB)
          LIN28 Antibody in Western Blot (WB)
          LIN28 Antibody in Immunoprecipitation (IP)

          Product Details

          11724-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:1,000
          View 23 publications 23 publications

          Immunohistochemistry (IHC)

          -
          View 18 publications 18 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 10 publications 10 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Dog, Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          LIN28 Fusion Protein Ag2312 (1-209 aa encoded by BC028566)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.31 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

          Immunogen sequence: MGSVSNQQF AGGCAKAAEE APEEAPEDAA RAADEPQLLH GAGICKWFNV RMGFGFLSMT ARAGVALDPP VDVFVHQSKL HMEGFRSLKE GEAVEFTFKK SAKGLESIRV TGPGGVFCIG SERRPKGKSM QKRRSKGDRC YNCGGLDHHA KECKLPPQPK KCHFCQSISH MVASCPLKAQ QGPSAQGKPT YFREEEEEIH SPTLLPEAQN (1-209 aa encoded by BC028566)

          Target Information

          Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: FLJ12457; Lin-28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1

          View more View less

          Gene Aliases: AL024421; CSDD1; ENSMUSG00000070700; Gm10299; LIN-28; lin-28A; LIN28; LIN28A; RGD1566408; Tex17; ZCCHC1

          View more View less

          UniProt ID: (Human) Q9H9Z2, (Mouse) Q8K3Y3

          View more View less

          Entrez Gene ID: (Human) 79727, (Rat) 500562, (Mouse) 83557

          View more View less

          Function(s)
          DNA binding RNA binding mRNA binding protein binding zinc ion binding translation initiation factor binding miRNA binding molecular_function nucleic acid binding metal ion binding
          Process(es)
          regulation of transcription, DNA-templated germ cell development biological_process miRNA metabolic process miRNA catabolic process stem cell population maintenance pre-miRNA processing RNA 3'-end processing positive regulation of neuron differentiation negative regulation of glial cell differentiation positive regulation of translation stem cell differentiation regulation of gene silencing by miRNA positive regulation of cell proliferation involved in kidney development somatic stem cell population maintenance gene silencing by RNA
          It has to be done as per old AB suggested Products section.
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