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LIN28B Antibody (PA5-50609) in WB
Western blot was performed using Anti-LIN28B Polyclonal Antibody (Product # PA5-50609) and a 35 kDa band corresponding to LIN28B was observed in BeWo, but not in SK-OV-3 and U-937. Modified whole cell lysates (1% SDS) (30 µg lysate) of BeWo (Lane 1), SK-OV-3 (Lane 2) and U-937 (Lane 3) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary... View More
Product Details
PA5-50609
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues near the C terminal of human lin-28 homolog B (C. elegans)
Conjugate
Form
Liquid
Concentration
1.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2636062
Product Specific Information
The antibody detects endogenous levels of total LIN28B protein.
Target Information
The GNL1 gene, identified in the human major histocompatibility complex class I region, shows a high degree of similarity with its mouse counterpart. The GNL1 gene is located less than 2 kb centromeric to HLA-E, in the same transcriptional orientation. GNL1 is telomeric to HLA-B and HLA-C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lin-28.2; Lin-28B; Protein lin-28 homolog B
Gene Aliases: 2810403D23Rik; CSDD2; D030047M17Rik; Lin-28.2; LIN28B
UniProt ID: (Human) Q6ZN17, (Mouse) Q45KJ6
Entrez Gene ID: (Human) 389421, (Mouse) 380669
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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