Invitrogen

LIN9 Polyclonal Antibody

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LIN9 Antibody (PA5-43640) in WB

Western blot analysis of human HeLa cell lysate using an anti-LIN9 polyclonal antibody (Product # PA5-43640).

Product Image Gallery

LIN9 Antibody — LIN9 mediates the effects of BET inhibition (A) Confirmation of siRNA-mediated knockdown of LIN9 in MDA-MB-231 cells after five days. Inset is a representative western blot showing suppression of LIN9 substantially reduces LIN9 protein. (B) RT-qPCR analysis of BETi-target genes in MDA-MB-231 cells following LIN9 silencing. (C) Representative images (20×) of MDA-MB-231 cells following LIN9 silencing that were stained with DAPI (blue, nuclei) and Texas Red-X phalloidin (red, actin cytoskeleton). MDA-MB-231 cells treated with 500 nM JQ1 are included as a comparison. Arrows indicate multinucleated cells. (D) Kaplan-Meier curve of relapse-free survival for breast cancer patients with high and low expression of LIN9. (E) Violin plots depicting BETi-induced expression (log2 fold change) of genes that are not correlated (r<0.5) or are correlated (r=0.5) with LIN9 expression. The right side of the panel depicts subdivision of genes that were correlated with LIN9 (r=0.5) according to residence on chromosome 1q versus their responsiveness to JQ1 (p-value for r<0.5 vs. r=0.5 = 1.8×10-47; p-value for r=0.5 on 1q vs. not on 1q = 1.2×10-5). r = Pearson’s coefficient. (F) Absolute fold change in expression following JQ1 treatment of genes that are highly correlated with LIN9 expression (r=0.5) and those that are moderately or not correlated (r<0.5) in the TCGA and METABRIC datasets. (G) Percent of genes changed with JQ1 that are bound by LIN9 or not bound by LIN9 (p<<0.001). For panels A, B, {KD}

Product Details

PA5-43640

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human LIN9 (aa 35-84).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607452

Product Specific Information

Peptide sequence: SLQKTPVWKG RNTSSAVEMP FRNSKRSRLF SDEDDRQINT RSPKRNQRVA

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

Target Information

LIN9 belongs to the lin-9 family and acts as a tumor suppressor. It inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. LIN9 plays a role in the expression of genes required for the G1/S transition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LIN9 and NEK2 Are Core Regulators of Mitotic Fidelity That Can Be Therapeutically Targeted to Overcome Taxane Resistance.

Cancer research

Roberts MS,Sahni JM,Schrock MS,Piemonte KM,Weber-Bonk KL,Seachrist DD,Avril S,Anstine LJ,Singh S,Sizemore ST,Varadan V,Summers MK,Keri RA

PA5-43640 was used in Western Blotting to demonstrate that the LIN9/NEK2 pathway is a therapeutically targetable mediator of taxane resistance that can be leveraged to improve response to this core chemotherapy.

Wed Apr 15 00:00:00 UTC 2020

Mitotic Vulnerability in Triple-Negative Breast Cancer Associated with LIN9 Is Targetable with BET Inhibitors.

Cancer research

Sahni JM,Gayle SS,Webb BM,Weber-Bonk KL,Seachrist DD,Singh S,Sizemore ST,Restrepo NA,Bebek G,Scacheri PC,Varadan V,Summers MK,Keri RA

Published figure using LIN9 polyclonal antibody (Product # PA5-43640) in Western Blot

Sun Oct 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Beta subunit-associated regulator of apoptosis; HuLin-9; lin-9 homolog; pRB-associated protein; Protein lin-9 homolog; rb related pathway actor; TUDOR gene similar protein; Type I interferon receptor beta chain-associated protein

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Gene Aliases: BARA; BARPsv; Lin-9; LIN9; TGS; TGS1; TGS2

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UniProt ID: (Human) Q5TKA1

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Entrez Gene ID: (Human) 286826

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Function(s)

protein binding

Process(es)

transcription, DNA-templated cell cycle regulation of cell cycle DNA biosynthetic process

Performance Guarantee

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LIN9 Polyclonal Antibody

LIN9 Antibody (PA5-43640) in WB

Product Details

PA5-43640

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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