Proteintech

LMNB2 Polyclonal Antibody

LMNB2 Antibody in Immunocytochemistry (ICC/IF)

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LMNB2 Antibody (10895-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10895-1-AP (LMNB2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

LMNB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LMNB2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10895-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LMNB2 Fusion Protein Ag1335 (241-600 aa encoded by BC006551)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EELRSQHDE QVRLYKLELE QTYQAKLDSA KLSSDQNDKA ASAAREELKE ARMRLESLSY QLSGLQKQAS AAEDRIRELE EAMAGERDKF RKMLDAKEQE MTEMRDVMQQ QLAEYQELLD VKLALDMEIN AYRKLLEGEE ERLKLSPSPS SRVTVSRATS SSSGSLSATG RLGRSKRKRL EVEEPLGSGP SVLGTGTGGS GGFHLAQQAS ASGSVSIEEI DLEGKFVQLK NNSDKDQSLG NWRIKRQVLE GEEIAYKFTP KYILRAGQMV TVWAAGAGVA HSPPSTLVWK GQSSWGTGES FRTVLVNADG EEVAMRTVKK SSVMRENENG EEEEEEAEFG EEDLFHQQGD PRTTSRGCYV M (241-600 aa encoded by BC006551)

Target Information

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-656-3p inhibits melanomas in vitro and in vivo by inducing senescence via inhibiting LMNB2.

Journal of cancer research and clinical oncology

Sun J,Lui K,Pang Q,Xu M,Zhao H,Shao J,Yu Y,Chu X,Liang Y,Xu J,Shen Z

Fri Sep 01 00:00:00 UTC 2023

Neutrophil S100A9 supports M2 macrophage niche formation in granulomas.

iScience

Mizutani T,Ano T,Yoshioka Y,Mizuta S,Takemoto K,Ouchi Y,Morita D,Kitano S,Miyachi H,Tsuruyama T,Fujiwara N,Sugita M

Fri Mar 17 00:00:00 UTC 2023

NBDHEX re-sensitizes adriamycin-resistant breast cancer by inhibiting glutathione S-transferase pi.

Cancer medicine

Sha H,Zou R,Lu Y,Gan Y,Ma R,Feng J,Chen D

Wed Mar 01 00:00:00 UTC 2023

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion.

Nature cell biology

Jung-Garcia Y,Maiques O,Monger J,Rodriguez-Hernandez I,Fanshawe B,Domart MC,Renshaw MJ,Marti RM,Matias-Guiu X,Collinson LM,Sanz-Moreno V,Carlton JG

Sun Jan 01 00:00:00 UTC 2023

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion.

Nature cell biology

Jung-Garcia Y,Maiques O,Monger J,Rodriguez-Hernandez I,Fanshawe B,Domart MC,Renshaw MJ,Marti RM,Matias-Guiu X,Collinson LM,Sanz-Moreno V,Carlton JG

Sun Jan 01 00:00:00 UTC 2023

Kruppel like factor 16 promotes lung adenocarcinoma progression by upregulating lamin B2.

Bioengineered

Jiao X,Gao W,Ren H,Wu Y,Li T,Li S,Yan H

Fri Apr 01 00:00:00 UTC 2022

Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.

Clinical and translational medicine

Wang L,Zhao L,Lin Z,Yu D,Jin M,Zhou P,Ren J,Cheng J,Yang K,Wu G,Zhang T,Zhang D

Sun May 01 00:00:00 UTC 2022

Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a.

Frontiers in oncology

Wan F,Zhang H,Hu J,Chen L,Geng S,Kong L,Lu JJ

Tue Mar 08 00:00:00 UTC 2022

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.

Aging

Zhang Z,Guo X,Guo X,Yu R,Qian M,Wang S,Gao X,Qiu W,Guo Q,Xu J,Chen Z,Wang H,Qi Y,Zhao R,Xue H,Li G

Mon Feb 01 00:00:00 UTC 2021

Antagonizing effects of curcumin against mercury-induced autophagic death and trace elements disorder by regulating PI3K/AKT and Nrf2 pathway in the spleen.

Ecotoxicology and environmental safety

Zhao G,Qi L,Wang Y,Li X,Li Q,Tang X,Wang X,Wu C

Fri Oct 01 00:00:00 UTC 2021

Dihydrotanshinone I Specifically Inhibits NLRP3 Inflammasome Activation and Protects Against Septic Shock In Vivo.

Frontiers in pharmacology

Wei Z,Zhan X,Ding K,Xu G,Shi W,Ren L,Fang Z,Liu T,Hou X,Zhao J,Li H,Li J,Li Z,Li Q,Lin L,Yang Y,Xiao X,Bai Z,Cao J

Tue Nov 02 00:00:00 UTC 2021

Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation.

Chemico-biological interactions

Fan Y,Xing Y,Xiong L,Wang J

Mon Jun 01 00:00:00 UTC 2020

LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma.

IUBMB life

Kong W,Wu Z,Yang M,Zuo X,Yin G,Chen W

Tue Dec 01 00:00:00 UTC 2020

HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.

Chemico-biological interactions

Dang X,Zhang R,Peng Z,Qin Y,Sun J,Niu Z,Pei H

Sat Jan 25 00:00:00 UTC 2020

Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signalling.

Journal of drug targeting

Wang M,Li C,Shi W

Wed Jul 01 00:00:00 UTC 2020

Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2.

European journal of immunology

Willebrand R,Dietschmann A,Nitschke L,Krappmann S,Voehringer D

Thu Nov 01 00:00:00 UTC 2018

Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2.

European journal of immunology

Willebrand R,Dietschmann A,Nitschke L,Krappmann S,Voehringer D

Thu Nov 01 00:00:00 UTC 2018

Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.

Oxidative medicine and cellular longevity

Ge M,Chen C,Yao W,Zhou S,Huang F,Cai J,Hei Z

Mon May 07 00:00:00 UTC 2018

Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects.

Oxidative medicine and cellular longevity

Ge M,Chen C,Yao W,Zhou S,Huang F,Cai J,Hei Z

Mon May 07 00:00:00 UTC 2018

HIV-1 Tat potently stabilises Mdm2 and enhances viral replication.

The Biochemical journal

Raja R,Ronsard L,Lata S,Trivedi S,Banerjea AC

Tue Jul 11 00:00:00 UTC 2017

HIV-1 Tat potently stabilises Mdm2 and enhances viral replication.

The Biochemical journal

Raja R,Ronsard L,Lata S,Trivedi S,Banerjea AC

Tue Jul 11 00:00:00 UTC 2017

Lamin B1 and lamin B2 are long-lived proteins with distinct functions in retinal development.

Molecular biology of the cell

Razafsky D,Ward C,Potter C,Zhu W,Xue Y,Kefalov VJ,Fong LG,Young SG,Hodzic D

Wed Jun 15 00:00:00 UTC 2016

Lamin B1 and lamin B2 are long-lived proteins with distinct functions in retinal development.

Molecular biology of the cell

Razafsky D,Ward C,Potter C,Zhu W,Xue Y,Kefalov VJ,Fong LG,Young SG,Hodzic D

Wed Jun 15 00:00:00 UTC 2016

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Oncogenesis

Kuga T,Nie H,Kazami T,Satoh M,Matsushita K,Nomura F,Maeshima K,Nakayama Y,Tomonaga T

Mon Mar 17 00:00:00 UTC 2014

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Oncogenesis

Kuga T,Nie H,Kazami T,Satoh M,Matsushita K,Nomura F,Maeshima K,Nakayama Y,Tomonaga T

Mon Mar 17 00:00:00 UTC 2014

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Oncogenesis

Kuga T,Nie H,Kazami T,Satoh M,Matsushita K,Nomura F,Maeshima K,Nakayama Y,Tomonaga T

Mon Mar 17 00:00:00 UTC 2014

Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation.

Oncogenesis

Kuga T,Nie H,Kazami T,Satoh M,Matsushita K,Nomura F,Maeshima K,Nakayama Y,Tomonaga T

Mon Mar 17 00:00:00 UTC 2014

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Experimental cell research

Kuga T,Nozaki N,Matsushita K,Nomura F,Tomonaga T

Sun Aug 15 00:00:00 UTC 2010

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Experimental cell research

Kuga T,Nozaki N,Matsushita K,Nomura F,Tomonaga T

Sun Aug 15 00:00:00 UTC 2010

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Experimental cell research

Kuga T,Nozaki N,Matsushita K,Nomura F,Tomonaga T

Sun Aug 15 00:00:00 UTC 2010

Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle.

Experimental cell research

Kuga T,Nozaki N,Matsushita K,Nomura F,Tomonaga T

Sun Aug 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: lamin B2; lamin B3; Lamin-B2; MGC2721

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Gene Aliases: EPM9; LAMB2; LMN2; LMNB2; RGD1563803

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UniProt ID: (Human) Q03252, (Mouse) P21619

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Entrez Gene ID: (Human) 84823, (Mouse) 16907, (Rat) 299625

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Function(s)

molecular_function structural molecule activity

Process(es)

biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LMNB2 Polyclonal Antibody

LMNB2 Antibody (10895-1-AP) in ICC/IF

Product Details

10895-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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