Invitrogen

LRP1 Monoclonal Antibody (A2MR-beta-1)

LRP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

LRP1 Antibody (37-7600) in ICC/IF

Immunofluorescence analysis of LRP1 was performed using 70% confluent log phase U-87 MG and SK-BR-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 2 hours at room temperature. The cells were labeled with LRP1 Monoclonal Antibody (A2MR-beta-1) (Product # 37-7600) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More

LRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LRP1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

LRP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

LRP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using LRP1 Monoclonal Antibody (A2MR-beta-1) (Product # 37-7600) shows expression of LRP1 in U-87-MG cell line in comparison to SK-BR-3. {RE}

Product Details

37-7600

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A2MR-beta-1

Immunogen

Synthetic peptide derived from the human CD91 beta chain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533340

Product Specific Information

37-7600 was used in IHC to successfully detect LRP-1 in mouse E18.5 lung section.

Target Information

The protein encoded by this gene is an endocytic receptor involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

37-7600 was used in Western Blotting and Immunoprecipitaiton to identify cell type-specific extracellular vesicle (EV) protein markers in human-induced pluripotent stem cell-derived neural cell types, including excitatory neurons (ATP1A3, NCAM1), astrocytes (LRP1, ITGA6), microglia-like cells (ITGAM, LCP1), and oligodendrocyte-like cells (LAMP2, FTH1), along with 16 pan-EV marker candidates.

Sat Jan 01 00:00:00 UTC 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

Sat Jan 01 00:00:00 UTC 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

Sat Jan 01 00:00:00 UTC 2022

LRP-1 receptor combines EGFR signalling and eHsp90α autocrine to support constitutive breast cancer cell motility in absence of blood supply.

Scientific reports

Chang C,Tang X,Mosallaei D,Chen M,Woodley DT,Schönthal AH,Li W

37-7600 was used in Western Blotting to uncover a novel mechanism by which certain breast cancer cells use lipoprotein receptor-related protein 1 to engage parallel signalling pathways to move when they lose contact with blood support.

Thu Jul 14 00:00:00 UTC 2022

ApoE3 vs. ApoE4 Astrocytes: A Detailed Analysis Provides New Insights into Differences in Cholesterol Homeostasis.

Antioxidants (Basel, Switzerland)

Staurenghi E,Leoni V,Lo Iacono M,Sottero B,Testa G,Giannelli S,Leonarduzzi G,Gamba P

37-7600 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to find that ApoE genotype clearly affects astrocytic cholesterol homeostasis.

Tue Nov 01 00:00:00 UTC 2022

Bio-nanoparticle based therapeutic vaccine induces immunogenic response against triple negative breast cancer.

American journal of cancer research

Bai X,Zhou Y,Lin Q,Huang CK,Zhang S,Carlson RI,Ghanbari H,Sun B,Wands JR,Dong X

37-7600 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to conclude that ? phage vaccine targeting a TAA (ASPH) combined with DOX successfully induces ICD and initiates CD8+ T cell-mediated antitumor immunity in breast tumors.

Tue May 07 00:00:00 UTC 2024

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Scientific reports

Dong H,Zou M,Bhatia A,Jayaprakash P,Hofman F,Ying Q,Chen M,Woodley DT,Li W

37-7600 was used in western blot to report that heat shock protein-90alpha allows cancer cells to survive hypoxia

Fri Feb 05 00:00:00 UTC 2016

Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Scientific reports

Dong H,Zou M,Bhatia A,Jayaprakash P,Hofman F,Ying Q,Chen M,Woodley DT,Li W

37-7600 was used in western blot to report that heat shock protein-90alpha allows cancer cells to survive hypoxia

Fri Feb 05 00:00:00 UTC 2016

Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Journal of cell science

Jayaprakash P,Dong H,Zou M,Bhatia A,O'Brien K,Chen M,Woodley DT,Li W

37-7600 was used in western blot to elucidate a role for heat shock proteins in ischemia.

Wed Apr 15 00:00:00 UTC 2015

Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Journal of cell science

Jayaprakash P,Dong H,Zou M,Bhatia A,O'Brien K,Chen M,Woodley DT,Li W

37-7600 was used in western blot to elucidate a role for heat shock proteins in ischemia.

Wed Apr 15 00:00:00 UTC 2015

Extracellular heat shock protein 90 signals through subdomain II and the NPVY motif of LRP-1 receptor to Akt1 and Akt2: a circuit essential for promoting skin cell migration in vitro and wound healing in vivo.

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 UTC 2013

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 UTC 2013

Molecular and cellular biology

Tsen F,Bhatia A,O'Brien K,Cheng CF,Chen M,Hay N,Stiles B,Woodley DT,Li W

37-7600 was used in immunoprecipitation and western blot to elucidate the mechanism by which eHsp90α regulates promotility action.

Sun Dec 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: A2MR; alpha 2-macroglobulin receptor; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; lipoprotein receptor-related protein; low density lipoprotein receptor-related protein 1; low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor); LRP-1; lrp1; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor

View more View less

Gene Aliases: A2MR; AI316852; APOER; APR; b2b1554Clo; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5

View more View less

UniProt ID: (Human) Q07954, (Mouse) Q91ZX7, (Rat) G3V928

View more View less

Entrez Gene ID: (Human) 4035, (Mouse) 16971, (Rat) 299858

View more View less

Function(s)

protease binding receptor activity low-density lipoprotein receptor activity calcium ion binding protein binding protein complex binding apolipoprotein binding lipoprotein transporter activity poly(A) RNA binding lipoprotein particle receptor binding metal ion binding

Process(es)

endocytosis receptor-mediated endocytosis protein kinase C-activating G-protein coupled receptor signaling pathway multicellular organism development cholesterol metabolic process cell proliferation positive regulation of cholesterol efflux negative regulation of neuron projection development negative regulation of smooth muscle cell migration negative regulation of Wnt signaling pathway positive regulation of lipid transport regulation of cholesterol transport regulation of phospholipase A2 activity regulation of actin cytoskeleton organization aorta morphogenesis apoptotic cell clearance negative regulation of neuron apoptotic process positive regulation of protein transport beta-amyloid clearance negative regulation of platelet-derived growth factor receptor-beta signaling pathway retinoid metabolic process aging cerebral cortex development lipoprotein metabolic process lipoprotein transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

LRP1 Monoclonal Antibody (A2MR-beta-1)

LRP1 Antibody (37-7600) in ICC/IF

Product Details

37-7600

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!