Invitrogen

LRP5 Polyclonal Antibody

LRP5 Antibody in Immunocytochemistry (ICC/IF)

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LRP5 Antibody (36-5400) in ICC/IF

Immunofluorescent staining of LRP5-transfected 293T cells using Rb anti-LRP5 (Product # 36-5400). Image courtesy of Heekyung Chung, PhD, and Anthony Brown, PhD, Weill Medical College of Cornell University, NY.

LRP5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

LRP5 Antibody — FIG. 5. Dkk1 treatment and LRP5/LRP6 siRNA stimulated neurite outgrowth. (A) Wnt-1 and Wnt-3a CM promoted beta-catenin stabilization in TC-32 cells. Stabilization of free beta-catenin was analyzed by GST-E-cadherin pull-down assay. Immunoblot (IB) analyses of beta-catenin pelleted with GST-E-cadherin and of beta-catenin and HSP70 in whole-cell lysates are indicated. -, absence of; +, presence of. (B) Dkk1 blocked Wnt-3a-induced beta-catenin stabilization in TC-32 cells. Cells were serum starved overnight and pretreated with Dkk1 for 30 min at 37degC in a CO 2 incubator. Wnt-3a CM or control L (Ctl.) CM were then added, and cells were further incubated for 3 h, followed by GST-E-cadherin pull-down assay. (C) Dkk1 induced neurite outgrowth. TC-32 cells plated on coverslips were pretreated with Dkk1 as indicated and subsequently incubated with Wnt-3a or control medium for 3 h. Then cells were fixed and stained with Alexa Fluor 568 phalloidin. Scale bars, 20 mum. (D) Quantitative analysis of the effect of Dkk1, Wnt-3a, and cotreatment with Dkk1 and Wnt-3a on neurite outgrowth. The percentage of cells having neurites was determined for each treatment group. The stimulation of neurite outgrowth for each group was defined as the ratio of this percentage to the percentage observed for the negative control. The results are means +- standard deviations (error bars) from three independent experiments. ***, P < 0.001. (E) Knockdown of LRP5 and LRP6 in TC-32 cells. Cells were transfected {KD}

Product Details

36-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533267

Target Information

LDL related proteins, LRP 5 and LRP 6, are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP 6 was shown to bind to Wnt 1 and enhance Wnt-induced developmental processes in Xenopus embryos. Mice lacking LRP 6 exhibited developmental defects that are similar to those caused by deficiencies in various Wnt proteins. Recent work also shows that Wnt proteins induce the binding of LRP 5 to Axin, leading Axin degradation and beta catenin stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Circular RNA circStag1 promotes bone regeneration by interacting with HuR.

Bone research

Chen G,Long C,Wang S,Wang Z,Chen X,Tang W,He X,Bao Z,Tan B,Zhao J,Xie Y,Li Z,Yang D,Xiao G,Peng S

36-5400 was used in Western Blot to explore the underlying functions of circRNAs in bone metabolism in postmenopausal osteoporosis.

Thu Mar 31 00:00:00 UTC 2022

Lrp4 expression by adipocytes and osteoblasts differentially impacts sclerostin's endocrine effects on body composition and glucose metabolism.

The Journal of biological chemistry

Kim SP,Da H,Li Z,Kushwaha P,Beil C,Mei L,Xiong WC,Wolfgang MJ,Clemens TL,Riddle RC

36-5400 was used in Western Blotting to investigate the contribution of Lrp4 to the endocrine function of sclerostin.

Fri Apr 26 00:00:00 UTC 2019

Lrp4 expression by adipocytes and osteoblasts differentially impacts sclerostin's endocrine effects on body composition and glucose metabolism.

The Journal of biological chemistry

Kim SP,Da H,Li Z,Kushwaha P,Beil C,Mei L,Xiong WC,Wolfgang MJ,Clemens TL,Riddle RC

36-5400 was used in Western Blotting to investigate the contribution of Lrp4 to the endocrine function of sclerostin.

Fri Apr 26 00:00:00 UTC 2019

Peptide-based mediated disruption of N-cadherin-LRP5/6 interaction promotes Wnt signaling and bone formation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Haÿ E,Buczkowski T,Marty C,Da Nascimento S,Sonnet P,Marie PJ

36-5400 was used in western blot to test if the intracellular domain of N-cadherin interacts with LRP5/6 to promote Wnt signaling and bone formation.

Sat Sep 01 00:00:00 UTC 2012

Peptide-based mediated disruption of N-cadherin-LRP5/6 interaction promotes Wnt signaling and bone formation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Haÿ E,Buczkowski T,Marty C,Da Nascimento S,Sonnet P,Marie PJ

36-5400 was used in western blot to test if the intracellular domain of N-cadherin interacts with LRP5/6 to promote Wnt signaling and bone formation.

Sat Sep 01 00:00:00 UTC 2012

Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells.

PloS one

Baljinnyam B,Klauzinska M,Saffo S,Callahan R,Rubin JS

36-5400 was used in western blot to investigate the contribution of Rspo/Wnt on gene expression

Mon May 14 00:00:00 UTC 2012

Wnt signaling mediates pathological vascular growth in proliferative retinopathy.

Circulation

Chen J,Stahl A,Krah NM,Seaward MR,Dennison RJ,Sapieha P,Hua J,Hatton CJ,Juan AM,Aderman CM,Willett KL,Guerin KI,Mammoto A,Campbell M,Smith LE

36-5400 was used in Western Blotting to demonstrate an important role of Wnt signalling in pathological vascular development in retinopathy and show a novel function of Cln5 in promoting angiogenesis.

Tue Oct 25 00:00:00 UTC 2011

Wnt signaling mediates pathological vascular growth in proliferative retinopathy.

Circulation

Chen J,Stahl A,Krah NM,Seaward MR,Dennison RJ,Sapieha P,Hua J,Hatton CJ,Juan AM,Aderman CM,Willett KL,Guerin KI,Mammoto A,Campbell M,Smith LE

Tue Oct 25 00:00:00 UTC 2011

Inactivation of nuclear Wnt-beta-catenin signaling limits blastocyst competency for implantation.

Development (Cambridge, England)

Xie H,Tranguch S,Jia X,Zhang H,Das SK,Dey SK,Kuo CJ,Wang H

36-5400 was used in Immunohistochemistry (PFA) to study the effects of silencing Wnt-beta-catenin signalling on blastocyst competency for implantation.

Fri Feb 01 00:00:00 UTC 2008

Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.

Molecular and cellular biology

Endo Y,Beauchamp E,Woods D,Taylor WG,Toretsky JA,Uren A,Rubin JS

Tue Apr 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: HGNC:6697; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; Low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 7; LRP-5; LRP-7

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Gene Aliases: BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP5; LRP7; mKIAA4142; OPPG; OPS; OPTA1; VBCH2

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UniProt ID: (Human) O75197, (Mouse) Q91VN0

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Entrez Gene ID: (Human) 4041, (Mouse) 16973

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Function(s)

protein binding Wnt-protein binding toxin transporter activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway

Process(es)

gastrulation with mouth forming second positive regulation of mesenchymal cell proliferation osteoblast development glucose catabolic process endocytosis cholesterol metabolic process regulation of blood pressure positive regulation of cell proliferation anterior/posterior pattern specification Wnt signaling pathway positive regulation of osteoblast proliferation somatic stem cell population maintenance extracellular matrix-cell signaling cell migration involved in gastrulation cholesterol homeostasis embryonic digit morphogenesis regulation of apoptotic process Wnt signaling pathway involved in dorsal/ventral axis specification positive regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter bone remodeling regulation of bone remodeling bone marrow development positive regulation of sequence-specific DNA binding transcription factor activity anatomical structure regression retina morphogenesis in camera-type eye canonical Wnt signaling pathway bone morphogenesis branching involved in mammary gland duct morphogenesis adipose tissue development cell-cell signaling involved in mammary gland development regulation of canonical Wnt signaling pathway regulation of insulin secretion involved in cellular response to glucose stimulus retinal blood vessel morphogenesis negative regulation of protein serine/threonine kinase activity apoptotic process involved in patterning of blood vessels beta-catenin destruction complex disassembly vasculature development multicellular organism development limb morphogenesis bone development mammary gland duct morphogenesis retina vasculature morphogenesis in camera-type eye

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LRP5 Polyclonal Antibody

LRP5 Antibody (36-5400) in ICC/IF

Product Details

36-5400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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