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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • LRP6 Antibodies

          Invitrogen

          LRP6 Polyclonal Antibody

          1 Reference
          View all (26) LRP6 antibodies

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          Datasheet
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          Datasheet
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          Cite LRP6 Polyclonal Antibody

          • Antibody Testing Data (3)
          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          LRP6 Antibody (PA5-89161) in ICC/IF

          Immunofluorescence analysis of LRP6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with LRP6 Polyclonal Antibody (Product # PA5-89161) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LRP6 Antibody in Immunocytochemistry (ICC/IF)
          LRP6 Antibody in Western Blot (WB)
          LRP6 Antibody in Western Blot (WB)

          Product Details

          PA5-89161

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human LRP6 (NP_0023272)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.14 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2805395

          Product Specific Information

          Immunogen sequence: APLLLYANRR DLRLVDATNG KENATIVVGG LEDAAAVDFV FSHGLIYWSD VSEEAIKRTE FNKTESVQNV VVSGLLSPDG LACDWLGEKL YWTDSETNRI EVSNLDGSLR KVLFWQELDQ PRAIALDPSS G; Positive Samples: Mouse liver; Cellular Location: Endoplasmic reticulum, Membrane, Single-pass type I membrane protein

          Target Information

          LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: crooked; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low density lipoprotein receptor-related protein 6; low density lipoprotein-related protein 6; low density lipoprotein-related protein 6; Low-density lipoprotein receptor-related protein 6; lpr6; LRP-6

          View more View less

          Gene Aliases: ADCAD2; C030016K15Rik; Cd; Gw; LRP6; ska26; ska; skax26; STHAG7

          View more View less

          UniProt ID: (Human) O75581, (Mouse) O88572

          View more View less

          Entrez Gene ID: (Human) 4040, (Mouse) 16974

          View more View less

          Function(s)
          low-density lipoprotein receptor activity receptor binding frizzled binding protein binding Wnt-protein binding kinase inhibitor activity toxin transporter activity apolipoprotein binding identical protein binding protein homodimerization activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway
          Process(es)
          neural tube closure negative regulation of protein phosphorylation pericardium morphogenesis negative regulation of protein kinase activity positive regulation of cytosolic calcium ion concentration chemical synaptic transmission embryonic pattern specification neural crest formation neural crest cell differentiation Wnt signaling pathway single organismal cell-cell adhesion cerebellum morphogenesis thalamus development cerebral cortex development midbrain-hindbrain boundary development negative regulation of smooth muscle cell apoptotic process external genitalia morphogenesis odontogenesis of dentin-containing tooth response to peptide hormone Wnt signaling pathway involved in dorsal/ventral axis specification canonical Wnt signaling pathway involved in neural crest cell differentiation canonical Wnt signaling pathway involved in regulation of cell proliferation positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity palate development convergent extension embryonic retina morphogenesis in camera-type eye canonical Wnt signaling pathway face morphogenesis trachea cartilage morphogenesis cellular response to cholesterol dopaminergic neuron differentiation negative regulation of protein serine/threonine kinase activity protein localization to plasma membrane primitive streak formation negative regulation of canonical Wnt signaling pathway receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport Wnt signaling pathway involved in somitogenesis axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway toxin transport beta-catenin destruction complex disassembly midbrain dopaminergic neuron differentiation Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification gastrulation with mouth forming second somitogenesis heart looping morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation axis elongation regulation of transcription, DNA-templated endocytosis receptor-mediated endocytosis multicellular organism development dorsal/ventral axis specification anterior/posterior pattern specification cerebral cortex cell migration forebrain radial glial cell differentiation forebrain generation of neurons Wnt signaling pathway involved in forebrain neuroblast division neural tube development formation of radial glial scaffolds negative regulation of Wnt signaling pathway embryonic limb morphogenesis forebrain development midbrain development limb morphogenesis embryonic forelimb morphogenesis embryonic hindlimb morphogenesis post-anal tail morphogenesis cell migration involved in gastrulation regulation of cell proliferation embryonic digit morphogenesis positive regulation of apoptotic process negative regulation of fat cell differentiation positive regulation of ossification positive regulation of bone resorption bone remodeling embryonic camera-type eye morphogenesis generation of neurons skeletal system morphogenesis negative regulation of epithelial cell proliferation response to folic acid retina morphogenesis in camera-type eye regulation of cell development bone morphogenesis branching involved in mammary gland duct morphogenesis mammary placode formation mammary gland duct morphogenesis canonical Wnt signaling pathway involved in cardiac neural crest cell differentiation involved in heart development canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation negative regulation of non-canonical Wnt signaling pathway negative regulation of planar cell polarity pathway involved in ventricular septum morphogenesis negative regulation of planar cell polarity pathway involved in cardiac muscle tissue morphogenesis negative regulation of planar cell polarity pathway involved in cardiac right atrium morphogenesis negative regulation of planar cell polarity pathway involved in outflow tract morphogenesis negative regulation of planar cell polarity pathway involved in pericardium morphogenesis negative regulation of planar cell polarity pathway involved in neural tube closure
          It has to be done as per old AB suggested Products section.
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