Invitrogen

LSD1 Monoclonal Antibody (K.884.2)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-14934

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:800
-

Immunocytochemistry (ICC/IF)

1:100-1:400
-

Immunoprecipitation (IP)

1:25
-

ChIP assay (ChIP)

5 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.884.2

Immunogen

Synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

17 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984411

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; neuroprotective protein 3; RP1-184J9.1

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Gene Aliases: 1810043O07Rik; AA408884; AOF2; BHC110; CPRF; D4Ertd478e; KDM1; KDM1A; KIAA0601; LSD1; mKIAA0601; RGD1562975

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UniProt ID: (Human) O60341, (Mouse) Q6ZQ88

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Entrez Gene ID: (Human) 23028, (Mouse) 99982, (Rat) 500569

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Function(s)
RNA polymerase II transcription factor binding p53 binding chromatin binding transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding oxidoreductase activity enzyme binding ligand-dependent nuclear receptor transcription coactivator activity demethylase activity histone demethylase activity histone demethylase activity (H3-K4 specific) histone demethylase activity (H3-K9 specific) histone demethylase activity (H3-dimethyl-K4 specific) telomeric DNA binding MRF binding transcription regulatory region DNA binding flavin adenine dinucleotide binding androgen receptor binding telomeric repeat-containing RNA binding DNA binding N-ethylmaleimide reductase activity reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity epoxyqueuosine reductase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein demethylation blood coagulation cell proliferation regulation of double-strand break repair via homologous recombination regulation of primitive erythrocyte differentiation histone deacetylation pituitary gland development granulocyte differentiation negative regulation of protein binding histone H3-K9 demethylation positive regulation of histone ubiquitination cellular response to UV histone H3-K4 demethylation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of erythrocyte differentiation positive regulation of megakaryocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation muscle cell development oxidation-reduction process cellular response to gamma radiation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of cellular protein localization positive regulation of neural precursor cell proliferation positive regulation of stem cell proliferation regulation of transcription, DNA-templated multicellular organism development positive regulation of neuron projection development chromatin modification positive regulation of chromatin binding positive regulation of cell size guanine metabolic process negative regulation of transcription from RNA polymerase II promoter by histone modification alternative mRNA splicing, via spliceosome response to organic cyclic compound cerebral cortex development neuron maturation response to fungicide cellular response to cAMP
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