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Invitrogen
LSM5 Polyclonal Antibody
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FIGURE: 1 / 14
LSM5 Antibody (PA5-144008) in ICC/IF
Immunocytochemistry analysis of LSM5 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM5 Polyclonal antibody (Product # PA5-144008) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
PA5-144008
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human LSM5 recombinant protein (Position: N8-V91).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075222
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human K562 whole cell, human MCF-7 whole cell. IHC: rat liver tissue, human appendicitis tissue, human rectal cancer tissue, human bladder cancer tissue, human lung cancer tissue, human ovary cancer tissue, human renal carcinoma tissue, human melanoma tissue. ICC/IF: MCF-7 cell. Flow: A431 cell, HEPA1-6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LSM5 homolog, U6 small nuclear RNA associated; LSM5 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated Sm-like protein LSm5
Gene Aliases: 2010208O10Rik; 2310034K10Rik; LSM5; YER146W
UniProt ID: (Human) Q9Y4Y9, (Mouse) P62322
Entrez Gene ID: (Human) 23658, (Mouse) 66373, (Rat) 306222
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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