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LSM7 Antibody (PA5-143997) in ICC/IF
Immunocytochemistry analysis of LSM7 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM7 Polyclonal antibody (Product # PA5-143997) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
PA5-143997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human LSM7 recombinant protein (Position: M1-A103).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075211
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human HEK293 whole cell, human MCF-7 whole cell, human Jurkat whole cell, human Raji whole cell, rat brain tissue, mouse brain tissue. IHC: human bladder cancer tissue, mouse brain tissue, rat brain tissue, mouse brain tissue, rat brain tissue, rat cerebellum tissue, human melanoma tissue, human ovary cancer tissue, human appendicitis tissue, human bladder cancer tissue, human gastric cancer tissue, human renal carcinoma tissue, human skin cancer tissue, human tonsil tissue, mouse cerebellum tissue. ICC/IF: MCF-7 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LSM7 homolog, U6 small nuclear RNA associated; LSM7 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated Sm-like protein LSm7
Gene Aliases: 0910001B06Rik; 1110033F18Rik; LSM7; YNL147W
UniProt ID: (Human) Q9UK45, (Mouse) Q9CQQ8
Entrez Gene ID: (Human) 51690, (Mouse) 66094, (Rat) 362829
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