Invitrogen

Laminin Polyclonal Antibody

9 Published Figures

24 References

View all (17) Laminin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (9)

Laminin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Laminin Antibody (PA1-16730) in ICC/IF

Immunocytochemistry analysis of Laminin in HeLa cells. Samples were incubated in Laminin polyclonal antibody (Product # PA1-16730) followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Laminin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Laminin Antibody in Immunohistochemistry - Free Floating (IHC (Free))

Laminin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Laminin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-16730

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:5,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:2,000

Immunohistochemistry - Free Floating (IHC (Free))

1:1,000-1:5,000

Immunocytochemistry (ICC/IF)

1:50-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Invertebrate, Mammal, Mouse, Sheep, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Laminin isolated from mouse EHS tumor.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2133633

Product Specific Information

This antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue. Suggested positive control: basement membranes (blood vessels of any tissue, tumors, nerve).

Target Information

Laminin antibodies are widely used to label blood vessels and basement membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Maternal e-cigarette use can disrupt postnatal blood-brain barrier (BBB) integrity and deteriorates motor, learning and memory function: influence of sex and age.

Fluids and barriers of the CNS

Archie SR,Sifat AE,Zhang Y,Villalba H,Sharma S,Nozohouri S,Abbruscato TJ

PA1-16730 was used in Western Blotting to suggest that prenatal e-cig exposure induces long-term neurovascular changes of neonates by disrupting postnatal BBB integrity and worsening behavioral outcomes.

Fri Mar 10 00:00:00 UTC 2023

Maternal e-cigarette use can disrupt postnatal blood-brain barrier (BBB) integrity and deteriorates motor, learning and memory function: influence of sex and age.

Fluids and barriers of the CNS

Archie SR,Sifat AE,Zhang Y,Villalba H,Sharma S,Nozohouri S,Abbruscato TJ

Fri Mar 10 00:00:00 UTC 2023

Quantitative evaluation of rat sciatic nerve degeneration using high-frequency ultrasound.

Scientific reports

Wu Y,Barrere V,Han A,Andre MP,Orozco E,Cheng X,Chang EY,Shah SB

PA1-16730 was used in Immunohistochemistry to suggest that backscatter coefficient spectrum-based and gray level co-occurrence matrices (GLCMs)-based analysis are promising non-invasive techniques for diagnosing peripheral nerve degeneration.

Sat Nov 18 00:00:00 UTC 2023

Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury.

Scientific reports

Huynh T,Reed C,Blackwell Z,Phelps P,Herrera LCP,Almodovar J,Zaharoff DA,Wolchok J

PA1-16730 was used in Immunohistochemistry to test the hypothesis that in addition to repairing the architectural and cellular cues via regenerative medicine, the delivery of immune cues (immunotherapy) may be needed to enhance regeneration following volumetric muscle loss (VML) injury.

Fri Feb 03 00:00:00 UTC 2023

Peripheral Nerve Decellularization for In Vitro Extracellular Matrix Hydrogel Use: A Comparative Study.

ACS biomaterials science & engineering

Gregory E,Baek IH,Ala-Kokko N,Dugan R,Pinzon-Herrera L,Almodóvar J,Song YH

PA1-16730 was used in Immunohistochemistry to establish a standard for peripheral nerve decellularization for usage as a dECM hydrogel testbed for in vitro peripheral nerve disease modeling.

Mon Jun 13 00:00:00 UTC 2022

Three-Dimensional Collagen Topology Shapes Cell Morphology, beyond Stiffness.

ACS biomaterials science & engineering

Chen C,Ibrahim Z,Marchand MF,Piolot T,Kamboj S,Carreiras F,Yamada A,Schanne-Klein MC,Chen Y,Lambert A,Aimé C

PA1-16730 was used in Immunocytochemistry to show that, while this phenotypic shift cannot be directly correlated with a unique ECM feature, the three-dimensional collagen fibril topology patterns cell shape, beyond protein composition and stiffness of the ECM.

Mon Dec 12 00:00:00 UTC 2022

ONOO^- -mediated oxidative modification of extracellular matrix aggravates atherosclerosis by affecting biological behaviors of vascular smooth muscle cells.

Cell biology international

Liang W,Liu Z,Zheng H,Lin A,Fan J,Li J,Wu W,Jie Q

PA1-16730 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to study the effect of oxidizing agent peroxynitrite (ONOO- )-mediated oxidization of ECM proteins on the biological behaviors of vascular smooth muscle cells (SMCs) and the development of AS.

Thu Sep 01 00:00:00 UTC 2022

Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection.

Fluids and barriers of the CNS

Endres LM,Jungblut M,Divyapicigil M,Sauer M,Stigloher C,Christodoulides M,Kim BJ,Schubert-Unkmeir A

PA1-16730 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to report for the first time, on co-culture of human iPSC derived BECs or hCMEC/D3 with meningioma derived LMCs and find that LMC co-culture improves barrier properties of iBECs.

Wed Oct 26 00:00:00 UTC 2022

Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old mice.

Science advances

Sanz-Ros J,Romero-García N,Mas-Bargues C,Monleón D,Gordevicius J,Brooke RT,Dromant M,Díaz A,Derevyanko A,Guío-Carrión A,Román-Domínguez A,Inglés M,Blasco MA,Horvath S,Viña J,Borrás C

PA1-16730 was used in Immunohistochemistry to propose that young sEV treatment can promote healthy aging.

Fri Oct 21 00:00:00 UTC 2022

Transcriptional and Epigenetic Consequences of DMSO Treatment on HepaRG Cells.

Cells

Dubois-Pot-Schneider H,Aninat C,Kattler K,Fekir K,Jarnouen K,Cerec V,Glaise D,Salhab A,Gasparoni G,Takashi K,Ishida S,Walter J,Corlu A

PA1-16730 was used in Immunocytochemistry to clarify Dimethyl sulfoxide influence on liver-specific gene expression.

Tue Jul 26 00:00:00 UTC 2022

Cancer Cell Invasion of Mammary Organoids with Basal-In Phenotype.

Advanced healthcare materials

Parigoris E,Lee S,Mertz D,Turner M,Liu AY,Sentosa J,Djomehri S,Chang HC,Luker K,Luker G,Kleer CG,Takayama S

PA1-16730 was used in Immunohistochemistry to describe mammary organoids with a basal-in phenotype where the basement membrane is located on the interior surface of the organoid.

Mon Feb 01 00:00:00 UTC 2021

Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin α in Glioblastoma Cells.

Frontiers in cell and developmental biology

Yu Q,Xiao W,Sun S,Sohrabi A,Liang J,Seidlits SK

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry (Paraffin)

Sun Apr 11 00:00:00 UTC 2021

Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin α in Glioblastoma Cells.

Frontiers in cell and developmental biology

Yu Q,Xiao W,Sun S,Sohrabi A,Liang J,Seidlits SK

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry (Paraffin)

Sun Apr 11 00:00:00 UTC 2021

Effect of Denervation on XBP1 in Skeletal Muscle and the Neuromuscular Junction.

International journal of molecular sciences

Walter LA,Blake LP,Gallot YS,Arends CJ,Sozio RS,Onifer SM,Bohnert KR

PA1-16730 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry - Free Floating, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that all three arms of the unfolded protein response (UPR) are activated in denervated skeletal muscle.

Fri Dec 24 00:00:00 UTC 2021

Effects of Aerobic Exercise Training on MyomiRs Expression in Cachectic and Non-Cachectic Cancer Mice.

Cancers

Gomes JLP,Tobias GC,Fernandes T,Silveira AC,Negrão CE,Chammas R,Brum PC,Oliveira EM

PA1-16730 was used in Immunohistochemistry to investigate the effects of AET on myomiRs expression in the skeletal muscle and serum of colon cachectic (CT26) and breast non-cachectic (MMTV-PyMT) cancer mice models.

Tue Nov 16 00:00:00 UTC 2021

Endothelial Lactate Controls Muscle Regeneration from Ischemia by Inducing M2-like Macrophage Polarization.

Cell metabolism

Zhang J,Muri J,Fitzgerald G,Gorski T,Gianni-Barrera R,Masschelein E,D'Hulst G,Gilardoni P,Turiel G,Fan Z,Wang T,Planque M,Carmeliet P,Pellerin L,Wolfrum C,Fendt SM,Banfi A,Stockmann C,Soro-Arnáiz I,Kopf M,De Bock K

PA1-16730 was used in Immunohistochemistry-immunofluorescence to conclude that ECs exploit glycolysis for angiocrine lactate shuttling to steer muscle regeneration from ischemia.

Tue Jun 02 00:00:00 UTC 2020

The effect of resistance training, detraining and retraining on muscle strength and power, myofibre size, satellite cells and myonuclei in older men.

Experimental gerontology

Blocquiaux S,Gorski T,Van Roie E,Ramaekers M,Van Thienen R,Nielens H,Delecluse C,De Bock K,Thomis M

PA1-16730 was used in Immunohistochemistry to investigate the role of myonuclei during training, detraining and retraining by using PCM1 labelling in muscle cross-sections of six older men.

Fri May 01 00:00:00 UTC 2020

Effect of natalizumab treatment on metalloproteinases and their inhibitors in a mouse model of multiple sclerosis.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society

Pyka-Fosciak G,Lis GJ,Litwin JA

PA1-16730 was used in Immunohistochemistry to assess whether natalizumab, a transmigration-inhibiting monoclonal antibody against α4β1 integrin, influences expression of MMPs and TIMPs in the central nervous system of mice with EAE.

Wed Apr 01 00:00:00 UTC 2020

Exercise promotes satellite cell contribution to myofibers in a load-dependent manner.

Skeletal muscle

Masschelein E,D'Hulst G,Zvick J,Hinte L,Soro-Arnaiz I,Gorski T,von Meyenn F,Bar-Nur O,De Bock K

PA1-16730 was used in Immunohistochemistry-immunofluorescence to study whether mechanical load during exercise independently promotes SC contribution to existing myofibers.

Thu Jul 09 00:00:00 UTC 2020

Exercise promotes satellite cell contribution to myofibers in a load-dependent manner.

Skeletal muscle

Masschelein E,D'Hulst G,Zvick J,Hinte L,Soro-Arnaiz I,Gorski T,von Meyenn F,Bar-Nur O,De Bock K

PA1-16730 was used in Immunohistochemistry-immunofluorescence to study whether mechanical load during exercise independently promotes SC contribution to existing myofibers.

Thu Jul 09 00:00:00 UTC 2020

Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.

EMBO reports

Wang T,Xu YQ,Yuan YX,Xu PW,Zhang C,Li F,Wang LN,Yin C,Zhang L,Cai XC,Zhu CJ,Xu JR,Liang BQ,Schaul S,Xie PP,Yue D,Liao ZR,Yu LL,Luo L,Zhou G,Yang JP,He ZH,Du M,Zhou YP,Deng BC,Wang SB,Gao P,Zhu XT,Xi QY,Zhang YL,Shu G,Jiang QY

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry

Sun Sep 01 00:00:00 UTC 2019

Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling.

EMBO reports

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry

Sun Sep 01 00:00:00 UTC 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA1-16730 was used in Flow cytometry/Cell sorting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 UTC 2019

Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study.

Journal of immunology research

Anghelescu VM,Neculae I,Dincă O,Vlădan C,Socoliuc C,Cioplea M,Nichita L,Popp C,Zurac S,Bucur A

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry

Thu Dec 27 00:00:00 UTC 2018

A semi-interpenetrating network of polyacrylamide and recombinant basement membrane allows pluripotent cell culture in a soft, ligand-rich microenvironment.

Biomaterials

Price AJ,Huang EY,Sebastiano V,Dunn AR

PA1-16730 was used in immunocytochemistry to develop a method to generate semi-interpenetrating networks of polyacrylamide and recombinant basement membrane to culture human embryonic stem cells

Wed Mar 01 00:00:00 UTC 2017

Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice.

The Journal of biological chemistry

Bal NC,Singh S,Reis FCG,Maurya SK,Pani S,Rowland LA,Periasamy M

Published figure using Laminin polyclonal antibody (Product # PA1-16730) in Immunohistochemistry

Fri Oct 06 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: dystrophia muscularis; Laminin A chain; laminin alpha 2 subunit; laminin B1; Laminin B1 chain; laminin B1 subunit 1; Laminin B2 chain; Laminin M chain; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit gamma-1; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, gamma 1 (formerly LAMB2); laminin-1; Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit alpha; Laminin-12 subunit beta; Laminin-2 subunit alpha; Laminin-2 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit alpha; Laminin-3 subunit gamma; Laminin-4 subunit alpha; Laminin-4 subunit gamma; Laminin-6 subunit beta; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit beta; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Merosin heavy chain; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma

View more View less

Gene Aliases: 5830440B04; AA408497; B2e; C77966; C80098; C81607; CLM; D130003D08Rik; dy; I79_015129; I79_017505; LAMA; Lama-1; LAMA1; LAMA2; Lamb-1; LAMB1; Lamb1-1; LAMB2; LAMC1; LAMM; LIS5; mer; merosin; mKIAA4087; PTBHS; S-LAM-alpha

View more View less

UniProt ID: (Human) P25391, (Human) P24043, (Human) P07942, (Human) P11047, (Mouse) P19137, (Mouse) Q60675, (Mouse) P02469

View more View less

Function(s)

receptor binding extracellular matrix structural constituent protein binding structural molecule activity glycosphingolipid binding molecular_function integrin binding enzyme binding

Process(es)

protein complex assembly cell adhesion endoderm development cell migration extracellular matrix disassembly extracellular matrix organization hemidesmosome assembly substrate adhesion-dependent cell spreading positive regulation of epithelial cell proliferation neuronal-glial interaction involved in cerebral cortex radial glia guided migration positive regulation of cell migration neuron projection development endodermal cell differentiation odontogenesis axon guidance muscle organ development myelination in peripheral nervous system regulation of cell adhesion regulation of cell migration positive regulation of synaptic transmission, cholinergic regulation of embryonic development negative regulation of cell adhesion embryo implantation learning or memory cell surface receptor signaling pathway biological_process morphogenesis of an epithelial sheet tissue development establishment of epithelial cell apical/basal polarity retina development in camera-type eye epithelial tube branching involved in lung morphogenesis branching involved in salivary gland morphogenesis retinal blood vessel morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Laminin Polyclonal Antibody

Laminin Antibody (PA1-16730) in ICC/IF

Product Details

PA1-16730

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!