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Invitrogen
Laminin beta-2/gamma-1 Monoclonal Antibody (M046)
FIGURE: 1 / 2
Laminin beta-2/gamma-1 Antibody (MA5-47722) in ICC/IF
Immunocytochemistry analysis of Laminin beta-2/gamma-1 in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse Laminin beta-2/gamma-1 monoclonal antibody (Product # MA5-47722). The antibody was detected using goat anti-mouse DyLight® 594.
Product Details
MA5-47722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
M046
Immunogen
Clone (M046) was generated from a recombinant human laminin 521 protein that includes a heterotrimer of three subunits laminin alpha5, laminin beta2, and laminin gamma1 subunits.
Conjugate
Form
Liquid
Concentration
~1.0 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942703
Product Specific Information
Clone M046 detects native laminin isoforms that include both laminin beta2 and gamma1 subunits. In native immunoblots, the antibody detects laminin 521, laminin 221, and laminin 121, but does not detect lamin 332 and laminin 411. The antibody weakly detects laminin 511. This antibody can be used for immunocytochemical detection of laminin beta2/gamma1 subunits, and can also be used for detection of native laminin isoforms in immunoblots and ELISA.
Target Information
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AA408497; Lama; Laminin B1s chain; Laminin B2 chain; laminin S; Laminin subunit beta-2; Laminin subunit gamma-1; laminin, beta 2 (laminin S); laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit beta; Laminin-11 subunit gamma; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit beta; Laminin-3 subunit gamma; Laminin-4 subunit beta; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit beta; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit beta; Laminin-9 subunit gamma; S-LAM beta; S-LAM gamma; S-laminin subunit beta; S-laminin subunit gamma
Gene Aliases: LAMB2; LAMC1; LAMS; NPHS5
UniProt ID: (Human) P55268, (Human) P11047
Entrez Gene ID: (Human) 3913, (Human) 3915
protein complex assembly
cell adhesion
endoderm development
cell migration
extracellular matrix disassembly
extracellular matrix organization
hemidesmosome assembly
substrate adhesion-dependent cell spreading
positive regulation of epithelial cell proliferation
axon guidance
neuromuscular junction development
visual perception
astrocyte development
Schwann cell development
axon extension involved in regeneration
retina development in camera-type eye
metanephric glomerular visceral epithelial cell development
metanephric glomerular basement membrane development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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