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          • Primary Antibodies ›
          • Laminin gamma-1 Antibodies

          Invitrogen

          Laminin gamma-1 Monoclonal Antibody (LN82-13)

          1 Reference
          View all (21) Laminin gamma-1 antibodies

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          Cite Laminin gamma-1 Monoclonal Antibody (LN82-13)

          Product Details

          33-5300

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          5-10 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Rabbit

          Published species

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          LN82-13

          Immunogen

          Human Placenta Laminin
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          2.0 mg/mL

          Purification

          Ion-exchange chromatography

          Storage buffer

          PBS, pH 7.4, with 1% BSA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533124

          Product Specific Information

          This antibody recognizes the N-terminal region of human laminin gamma 1 chain. This antibody does not inhibit cell adhesion.

          This antibody reacts with rabbit laminin. This antibody does not react with mouse laminin.

          Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with human placenta laminin.

          Reconstitution: Dissolve the lyophilized antibody in 50 mL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 mL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

          Storage : 4°C, This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

          Application: Western blot analysis under non-reducing and non-heating condition (can not be used under reducing condition). Immunohistochemical detection of laminin on frozen or paraffin embedded tissue sections.

          Target Information

          Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Laminin B2 chain; Laminin subunit gamma-1; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; S-LAM gamma; S-laminin subunit gamma

          View more View less

          Gene Aliases: LAMB2; LAMC1

          View more View less

          UniProt ID: (Human) P11047

          View more View less

          Entrez Gene ID: (Human) 3915

          View more View less

          Function(s)
          extracellular matrix structural constituent
          Process(es)
          protein complex assembly cell adhesion endoderm development cell migration extracellular matrix disassembly extracellular matrix organization hemidesmosome assembly substrate adhesion-dependent cell spreading positive regulation of epithelial cell proliferation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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