Invitrogen

Leptin Polyclonal Antibody

Leptin Antibody in Immunocytochemistry (ICC/IF)

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Leptin Antibody (PA1-051) in ICC/IF

Immunofluorescence analysis of Leptin was performed using 70% confluent log phase 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Leptin Rabbit Polyclonal Antibody (Product # PA1-051) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG... View More

Leptin Antibody in Immunohistochemistry (IHC)

Leptin Antibody in Western Blot, ELISA (WB, ELISA)

Leptin Antibody in Immunomicroscopy (IM)

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Product Details

PA1-051

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50 - 1:200

Immunocytochemistry (ICC/IF)

2 µg/mL

ELISA (ELISA)

View 1 publication 1 publication

ChIP assay (ChIP)

1.5 µg/1x10^6 cells

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325786

Product Specific Information

PA1-051 detects leptin from human, mouse, sheep and rat samples.

PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.

The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Evidence-based complementary and alternative medicine : eCAM

Cao YJ,Li HZ,Zhao J,Sun YM,Jin XW,Lv SQ,Luo JY,Fang XX,Wen WB,Liao JB

PA1-051 was used in Western Blotting to investigate the mechanisms by which Jian-Gan-Xiao-Zhi decoction improve nonalcoholic fatty liver disease.

Tue Jul 19 00:00:00 UTC 2022

Palmitate-induced Endoplasmic Reticulum stress and subsequent C/EBPα Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain.

Cellular signalling

Marwarha G,Claycombe K,Schommer J,Collins D,Ghribi O

PA1051 was used in western blot to propose that ER stress-induced CHOP activation in the brain links palmitate-induced regulation of leptin and IGF1

Tue Nov 01 00:00:00 UTC 2016

Palmitate-induced Endoplasmic Reticulum stress and subsequent C/EBPα Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain.

Cellular signalling

Marwarha G,Claycombe K,Schommer J,Collins D,Ghribi O

PA1051 was used in western blot to propose that ER stress-induced CHOP activation in the brain links palmitate-induced regulation of leptin and IGF1

Tue Nov 01 00:00:00 UTC 2016

Endoplasmic reticulum stress-induced CHOP activation mediates the down-regulation of leptin in human neuroblastoma SH-SY5Y cells treated with the oxysterol 27-hydroxycholesterol.

Cellular signalling

Marwarha G,Dasari B,Ghribi O

PA1-051 was used in western blot to investigate the mechanism of leptin down-regulation in 27-hydroxycholesterol-treated human neuroblastoma cells

Wed Feb 01 00:00:00 UTC 2012

Pressure mediated hypertrophy and mechanical stretch up-regulate expression of the long form of leptin receptor (ob-Rb) in rat cardiac myocytes.

BMC cell biology

Matsui H,Yokoyama T,Tanaka C,Sunaga H,Koitabashi N,Takizawa T,Arai M,Kurabayashi M

PA1-051 was used in western blot to study the upregulation of cardiac expression of the long form of the leptin receptor in response to pressure overload hypertrophy and mechanical stretch

Thu Dec 27 00:00:00 UTC 2012

Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer's disease.

Journal of Alzheimer's disease : JAD

Greco SJ,Bryan KJ,Sarkar S,Zhu X,Smith MA,Ashford JW,Johnston JM,Tezapsidis N,Casadesus G

PA1-051 was used in western blot to investigate the effect of leptin treatment on histopathology and memory capability in Alzheimer disease model

Thu Jul 22 00:00:00 UTC 2010

Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hansen GH,Niels-Christiansen LL,Danielsen EM

PA1-051 was used in immunohistochemistry and western blot to study the localization of the leptin and the obesity receptor in the small intestine and colon.

Tue Jul 01 00:00:00 UTC 2008

Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hansen GH,Niels-Christiansen LL,Danielsen EM

PA1-051 was used in immunohistochemistry and western blot to study the localization of the leptin and the obesity receptor in the small intestine and colon.

Tue Jul 01 00:00:00 UTC 2008

Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hansen GH,Niels-Christiansen LL,Danielsen EM

PA1-051 was used in immunohistochemistry and western blot to study the localization of the leptin and the obesity receptor in the small intestine and colon.

Tue Jul 01 00:00:00 UTC 2008

Leptin is synthesized in the liver and adipose tissue of the dunlin (Calidris alpina).

General and comparative endocrinology

Kochan Z,Karbowska J,Meissner W

PA1-051 was used in western blot to investigate the localization of leptin expression in the dunlin

Fri Sep 15 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB-R; Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein

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Gene Aliases: LEP; LEPD; OB; obese; OBS

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UniProt ID: (Human) P41159, (Rat) P50596, (Mouse) P41160

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Entrez Gene ID: (Sheep) 443534, (Human) 3952, (Rat) 25608, (Mouse) 16846

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Function(s)

hormone activity growth factor activity peptide hormone receptor binding cytokine activity protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Leptin Polyclonal Antibody

Leptin Antibody (PA1-051) in ICC/IF

Product Details

PA1-051

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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