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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Leptin Antibodies

          Invitrogen

          Leptin Polyclonal Antibody

          1 Published Figure
          12 References
          View all (34) Leptin antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Leptin Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (1)
          Leptin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Leptin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Leptin Antibody (PA1-052) in ICC/IF

          Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Leptin polyclonal antibody (Product # PA1-052) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-ac... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Leptin Antibody in Immunocytochemistry (ICC/IF)
          Leptin Antibody in Immunocytochemistry (ICC/IF)
          Leptin Antibody in Immunocytochemistry (ICC/IF)
          Leptin Antibody in Western Blot (WB)
          Leptin Antibody in Western Blot (WB)

          Product Details

          PA1-052

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          View 7 publications 7 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig

          Published species

          Human, Mouse, Pig, Sheep

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325787

          Product Specific Information

          PA1-052 detects leptin from human, mouse, and porcine samples.

          PA1-052 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~16 kDa protein representing leptin from human serum.

          The PA1-052 immunogen is a synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin. This sequence is 94% conserved between human and bovine.

          Target Information

          Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB-R; obese; Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein

          View more View less

          Gene Aliases: LEP; LEPD; OB; obese; OBS

          View more View less

          UniProt ID: (Pig) Q29406, (Human) P41159, (Mouse) P41160

          View more View less

          Entrez Gene ID: (Pig) 396832, (Human) 3952, (Mouse) 16846

          View more View less

          Function(s)
          hormone activity growth factor activity peptide hormone receptor binding protein binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovulation from ovarian follicle response to hypoxia positive regulation of cytokine production placenta development regulation of endothelial cell proliferation response to dietary excess cardiac muscle hypertrophy glucose metabolic process regulation of gluconeogenesis energy reserve metabolic process glycerol biosynthetic process lipid metabolic process fatty acid beta-oxidation tyrosine phosphorylation of STAT protein female pregnancy circadian rhythm cholesterol metabolic process bile acid metabolic process regulation of blood pressure adult feeding behavior negative regulation of autophagy negative regulation of lipid storage positive regulation of phosphatidylinositol 3-kinase signaling response to activity sexual reproduction central nervous system neuron development insulin secretion T cell differentiation regulation of intestinal cholesterol absorption positive regulation of TOR signaling negative regulation of appetite prostaglandin secretion response to estradiol regulation of natural killer cell activation regulation of natural killer cell proliferation response to insulin response to vitamin E leptin-mediated signaling pathway positive regulation of luteinizing hormone secretion positive regulation of peroxisome proliferator activated receptor signaling pathway bone mineralization involved in bone maturation negative regulation of appetite by leptin-mediated signaling pathway positive regulation of T cell proliferation regulation of natural killer cell mediated cytotoxicity hormone metabolic process positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis eating behavior negative regulation of apoptotic process positive regulation of ion transport positive regulation of MAPK cascade cellular response to leptin stimulus response to ethanol positive regulation of myeloid cell differentiation regulation of angiogenesis negative regulation of vasoconstriction negative regulation of glucose import positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway regulation of bone remodeling positive regulation of follicle-stimulating hormone secretion positive regulation of developmental growth regulation of insulin secretion regulation of steroid biosynthetic process intestinal absorption leukocyte tethering or rolling regulation of nitric-oxide synthase activity regulation of cell cycle positive regulation of protein kinase B signaling regulation of lipoprotein lipid oxidation adipose tissue development negative regulation of cartilage development negative regulation of glucagon secretion cellular response to L-ascorbic acid cellular response to retinoic acid interleukin-6 secretion interleukin-8 secretion regulation of brown fat cell differentiation bone growth regulation of cytokine production involved in inflammatory response positive regulation of p38MAPK cascade positive regulation of fat cell apoptotic process activation of protein kinase C activity positive regulation of STAT protein import into nucleus positive regulation of reactive oxygen species metabolic process negative regulation of glutamine transport positive regulation of hepatic stellate cell activation regulation of protein phosphorylation signal transduction positive regulation of cell proliferation fatty acid catabolic process negative regulation of metabolic process regulation of metabolic process intracellular signal transduction positive regulation of protein import into nucleus regulation of fat cell differentiation regulation of protein localization to nucleus
          It has to be done as per old AB suggested Products section.
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