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Invitrogen
Leptin Receptor Recombinant Rabbit Monoclonal Antibody (JA73-01)
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FIGURE: 1 / 7
Leptin Receptor Antibody (MA5-32685) in ICC/IF
Immunocytochemical analysis of Leptin Receptor in MCF-7 cells using a Leptin Receptor Monoclonal antibody (Product # MA5-32685) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA73-01
Immunogen
Synthetic peptide within Human Leptin Receptor aa 1041-1090
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809962
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B219; CD antigen CD295; CD295; CD295 antigen; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R
Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR
UniProt ID: (Human) P48357, (Rat) Q62959, (Mouse) P48356
Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847
angiogenesis
ovulation from ovarian follicle
response to hypoxia
generation of precursor metabolites and energy
G-protein coupled receptor signaling pathway
female pregnancy
response to nutrient
cholesterol metabolic process
cytokine-mediated signaling pathway
leptin-mediated signaling pathway
response to nicotine
eating behavior
positive regulation of MAPK cascade
response to estrogen
response to leptin
negative regulation of gluconeogenesis
negative regulation of hydrolase activity
cellular response to organic substance
energy reserve metabolic process
cell surface receptor signaling pathway
multicellular organism development
negative regulation of autophagy
sexual reproduction
T cell differentiation
glucose homeostasis
regulation of bone remodeling
regulation of feeding behavior
energy homeostasis
bone growth
regulation of energy homeostasis
signal transduction
regulation of metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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