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Invitrogen
Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor™ Yellow 660, eBioscience™
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FIGURE: 1 / 3
Ly-108 Antibody (M050T03Y04-A) in Flow
C57BL/6 mouse splenocytes were unstained (left) or stained with 0.8 µg of Ly-108 Monoclonal Antibody, NovaFluor Yellow 660 (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
M050T03Y04-A
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
eBio13G3-19D (13G3-19D)
Conjugate
Excitation/Emission Max
550/664 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
RRID
AB_3099213 (AB_2931054)
Product Specific Information
Description: The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.
Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.8 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow) Laser
Our internal testing shows that NovaFluor Yellow 660 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.
Target Information
Ly108 is the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is expressed on NK, B cells, and T cells. Activation of Ly108 receptor enhances NK cell activity and IFN-gamma expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD352; Lymphocyte antigen 108; SLAM family member 6
Gene Aliases: KAL1; KAL1b; Ly108; NTB-A; NTBA; SF2000; Slamf6
UniProt ID: (Mouse) Q9ET39
Entrez Gene ID: (Mouse) 30925
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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