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Invitrogen
Ly-49A Monoclonal Antibody (A1 (Ly49A)), PE, eBioscience™
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FIGURE: 1 / 3
Ly-49A Antibody (12-5856-82) in Flow
Staining of CD3- (Product # 11-0031-82) C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or 0.25 µg of Anti-Mouse Ly-49A PE (right).
Product Details
12-5856-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
A1 (Ly49A)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1311270
Product Specific Information
Description: This A1 monoclonal antibody reacts with Ly49A, a 44-kDa member of the Ly49 family of type II transmembrane receptors expressed on natural killer (NK) cells. This receptor is present as a disulfide-linked homodimer with C-type lectin extracellular domains. An inhibitory receptor, Ly49A binds MHC class I molecules, such as H-2Dd, H-2Dk, and H-2DP, to inhibit NK-mediated cytotoxicity by triggering phosphorylation of its ITIM domain and recruitment of SHP-1 and SHP-2. Ly49A is expressed on DX5+CD3- NK cells and DX5+CD3+ NK T cells, as well as on a population of memory CD8+ T cells in C57BL/6, C57BL/10, and B10 congenic mice.
Applications Reported: This A1 (Ly49A) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This A1 (Ly49A) antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ly49A is a gene that encodes a protein expressed on the surface of natural killer cells, which is involved in recognizing and eliminating virally infected or cancerous cells. It is located on chromosome 6 and is part of a larger family of genes that encode for similar receptors. The Ly49A protein interacts with a variety of ligands, including certain viruses and tumor cells, and is involved in regulating the activation and function of natural killer cells. Mutations or variations in the Ly49A gene have been associated with various diseases and conditions, including autoimmune disorders and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: class I MHC receptor KLRA22; KLRA#; ly-49a; lymphocyte antigen 49a; natural killer cell receptor Ly49A; novel killer cell lectin-like receptor subfamily A protein; t lymphocyte antigen A1; T-cell surface glycoprotein YE1/48
Gene Aliases: A1; CH29-493D4.3; Klra22; Ly49a; Ly49o<129>; Ly49v
Entrez Gene ID: (Mouse) 16627
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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