Invitrogen

Ly-49C/I/F/H Monoclonal Antibody (14B11), PE, eBioscience™

Ly-49C/I/F/H Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Ly-49C/I/F/H Antibody (12-5991-81) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Anti-Mouse Ly-49C/I/F/H PE (right). Total viable cells were used for analysis.

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Product Details

12-5991-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

14B11

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466106

Product Specific Information

Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.

Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

12-5991 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 UTC 2019

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

12-5991 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 UTC 2016

Lysophosphatidylcholine plays critical role in allergic airway disease manifestation.

Scientific reports

Bansal P,Gaur SN,Arora N

12-5991 was used in Flow cytometry/Cell sorting to show that lysophosphatidylcholine induces allergic airway disease in a mouse model, likely via CD1d-restricted LY49C(+)TCRβ(+) natural killer T cells.

Fri Jun 10 00:00:00 UTC 2016

Gammadelta T lymphocyte homeostasis is negatively regulated by beta2-microglobulin.

Journal of immunology (Baltimore, Md. : 1950)

French JD,Roark CL,Born WK,O'Brien RL

12-5991 was used in Flow cytometry/Cell sorting to investigate the role of β2-microglobulin in regulating gamma-delta T lymphocyte homeostasis.

Sun Feb 15 00:00:00 UTC 2009

Tumor growth impedes natural-killer-cell maturation in the bone marrow.

Blood

Richards JO,Chang X,Blaser BW,Caligiuri MA,Zheng P,Liu Y

12-5991 was used in Flow cytometry/Cell sorting to demonstrate that tumour growth can impede functional maturation of NK cells, most likely by interrupting the requisite IL-15 signalling pathway.

Sat Jul 01 00:00:00 UTC 2006

KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice.

The Journal of clinical investigation

Parsons CH,Adang LA,Overdevest J,O'Connor CM,Taylor JR,Camerini D,Kedes DH

12-5991 was used in Flow cytometry/Cell sorting to develop an animal model for Kaposi sarcoma-associated herpesvirus (KSHV) infection.

Sat Jul 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: 5E6; cytomegalovirus resistance 1; Killer cell lectin-like receptor 3; Killer cell lectin-like receptor 6; Killer cell lectin-like receptor 8; Ly-49c; Ly-49f; Ly-49h; Lymphocyte antigen 49c; Lymphocyte antigen 49f; Lymphocyte antigen 49h; natural killer cell-associated antigen 2; Nk2.1; T-cell surface glycoprotein Ly-49C; T-cell surface glycoprotein Ly-49F; T-cell surface glycoprotein Ly-49H

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Gene Aliases: 5E6; Cmv-1; Cmv1; Klra3; Klra6; Klra8; Ly-49c; Ly-49f; Ly-49h; Ly49-f; Ly49-h; Ly49C; Ly49F; Ly49H; Ly49u<129>; Nk-2; NK-2.1; Nk2; Nk2.1

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UniProt ID: (Mouse) Q64329, (Mouse) Q60653, (Mouse) Q60682

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Entrez Gene ID: (Mouse) 16634, (Mouse) 16637, (Mouse) 16639

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Function(s)

carbohydrate binding

Process(es)

cell adhesion response to virus

Performance Guarantee

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Ly-49C/I/F/H Monoclonal Antibody (14B11), PE, eBioscience™

Ly-49C/I/F/H Antibody (12-5991-81) in Flow

Product Details

12-5991-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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