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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Ly-6A/E (Sca-1) Antibodies

          Invitrogen

          Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™

          93 Published Figures
          95 References
          View all (33) Ly-6A/E (Sca-1) antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (93)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 94

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          Ly-6A/E (Sca-1) Antibody (62-5981-82) in Flow

          Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1172-82) and 0.125 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436 (right). Cells in the large scatter population were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry (ICC/IF)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry (ICC/IF)
          Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
          Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
          Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
          Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
          Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)
          Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)

          Product Details

          62-5981-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunocytochemistry (ICC/IF)

          -
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 84 publications 84 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Fish, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          D7

          Conjugate

          Super Bright™ 436 Super Bright™ 436 Super Bright™ 436
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 711
          • Brilliant Violet 786
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          413/431 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2637287

          Product Specific Information

          Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

          Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

          View more View less

          Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

          View more View less

          UniProt ID: (Mouse) P05533, (Mouse) Q64253

          View more View less

          Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

          View more View less

          Process(es)
          in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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