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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Ly-6C Antibodies

          Invitrogen

          Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™

          64 Published Figures
          70 References
          View all (15) Ly-6C antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor™ 780, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (64)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Ly-6C Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 65

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          Ly-6C Antibody (47-5932-82) in Flow

          Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.25 µg of Anti-Mouse Ly-6C APC-eFluor® 780. Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)
          Ly-6C Antibody in Flow Cytometry (Flow)

          Product Details

          47-5932-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 70 publications 70 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2c, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          HK1.4

          Conjugate

          APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780
          • Alexa Fluor 488 
          • APC 
          • eFluor 450
          • Functional Grade
          • PE 
          • PE-Cyanine7
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Request custom conjugation

          Excitation/Emission Max

          756/785 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573992

          Product Specific Information

          Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.

          In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.

          Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

          Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; Lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C

          View more View less

          Gene Aliases: AA682074; AA959465; Ly-6C; Ly-6C1; Ly6c; Ly6c1

          View more View less

          UniProt ID: (Mouse) P0CW02

          View more View less

          Entrez Gene ID: (Mouse) 17067

          View more View less

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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