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Invitrogen
Ly-6K Monoclonal Antibody (B33fc8k), PE, eBioscience™
FIGURE: 1 / 1
Ly-6K Antibody (12-5929-82) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse CD23 eFluor® 660 (Product # 50-0232-82), Anti-Mouse IgM PE-Cy7 (Product # 25-5790-82) and 0.5 µg of Anti-Mouse Ly-6K PE. Staining of Ly-6K (green) and CD23 (purple) is subgated to show expression levels of IgM (right).
Product Details
12-5929-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B33fc8k
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11149356
Product Specific Information
Description: This B33fc8k monoclonal antibody reacts with mouse Ly-6k, a member of the Ly6/uPAR superfamily. Ly-6k is a GPI linked protein predominantly found on the cell surface. One study suggests expression may also be non-membranous. Expression is found on B cells and reported to be on plasma cells as well as cells in the testis. Using the B33fc8k monoclonal antibody, expression is on a B lymphocyte cell subset in the spleen that correlates to transitional cells (CD23-IgM-). In humans Ly-6k has been found associated with carcinomas of the head and testis.
Applications Reported: This B33fc8k antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B33fc8k antibody has been tested by flow cytometric analyis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida. May play a role in cell growth (PubMed:18089789). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ35226; Ly-6K; Lymphocyte antigen 6K
Gene Aliases: 2410015A16Rik; 3110035B01Rik; Ly6k; mLy-6K
UniProt ID: (Mouse) Q9CWP4
Entrez Gene ID: (Mouse) 76486
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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