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Invitrogen
Lysozyme Monoclonal Antibody (BGN/0696/5B1)
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FIGURE: 1 / 3
Lysozyme Antibody (MA1-82873) in ICC/IF
Immunofluorescence analysis of Lysozyme was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Lysozyme Mouse Monoclonal Antibody (BGN/0696/5B1) (Product # MA1-82873) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluo... View More
Product Details
MA1-82873
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BGN/0696/5B1
Immunogen
Native protein, from urine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_934526
Product Specific Information
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control for this product is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human Lysozyme antibody, clone BGN/0696/5B1 recognizes lysozyme, an enzyme that is present in mucosal secretions such as saliva and tears and is involved with non-specific organism defence.
Target Information
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1,4-beta-N-acetylmuramidase C; c-type lysozyme; Lysozyme C; lysozyme F1
Gene Aliases: LYZ; LYZF1; LZM
UniProt ID: (Human) P61626
Entrez Gene ID: (Human) 4069
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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