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M-RAS/Mras Antibody (BS-1882R) in WB
Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MRas Polyclonal Antibody, Unconjugated (bs-1882R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product Details
BS-1882R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human MRAS, amino acids 105-208.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MRAS is a member of the RAS superfamily of GTP-binding proteins which are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. MRAS is oncogenically activated in a significant fraction of tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Muscle and microspikes Ras; Ras-related protein M-Ras; Ras-related protein R-Ras3; X-Ras
Gene Aliases: 2900078C09Rik; AI326250; M-RAs; MRAS; R-RAS3; RRAS3; Xras
UniProt ID: (Human) O14807, (Mouse) O08989
Entrez Gene ID: (Human) 22808, (Mouse) 17532, (Rat) 25482
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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