Invitrogen

MAD2 Polyclonal Antibody

MAD2 Antibody in Immunocytochemistry (ICC/IF)

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MAD2 Antibody (PA5-21594) in ICC/IF

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody (Product # PA5-21594) at 1:1,000 dilution.

MAD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MAD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

MAD2 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with MAD2 shRNA and decrease in signal intensity was observed in western blot application using MAD2 antibody (Product # PA5-21594). {KD}

Product Details

PA5-21594

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.01 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154021

Product Specific Information

Recommended positive controls: 293T, NCIN87, U2OS, HepG2, Molt4, Raji.

Predicted reactivity: Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.

Oncogene

Singh A,Busacca S,Gaba A,Sheaff M,Poile C,Nakas A,Dzialo J,Bzura A,Dawson AG,Fennell DA,Fry AM

PA5-21594 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to propose that BRCA1-associated protein 1 inactivation causes mitotic defects through BRCA1-dependent and independent mechanisms.

Wed Feb 01 00:00:00 UTC 2023

BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.

Oncogene

Singh A,Busacca S,Gaba A,Sheaff M,Poile C,Nakas A,Dzialo J,Bzura A,Dawson AG,Fennell DA,Fry AM

Wed Feb 01 00:00:00 UTC 2023

CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora B.

Cell death & disease

Ryu J,Kim JE

Published figure using MAD2 polyclonal antibody (Product # PA5-21594) in Immunocytochemistry

Tue Jun 07 00:00:00 UTC 2022

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

The Journal of cell biology

Kumari A,Kumar C,Pergu R,Kumar M,Mahale SP,Wasnik N,Mylavarapu SVS

PA5-21594 was used in Western Blotting to reveal that LIC1-CTD phosphorylation differentially regulates distinct mitotic dynein pools and suggests the evolutionary conservation of this phosphoregulation.

Mon Dec 06 00:00:00 UTC 2021

Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest.

The EMBO journal

Allan LA,Skowyra A,Rogers KI,Zeller D,Clarke PR

PA5-21594 was used in Western Blotting to show that proteolytic destruction of the key regulator Mcl-1 during an extended mitosis requires the anaphase-promoting complex or cyclosome (APC/C) and is independent of another ubiquitin E3 ligase, SCFFbw7.

Mon Sep 03 00:00:00 UTC 2018

TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.

British journal of pharmacology

Choi M,Min YH,Pyo J,Lee CW,Jang CY,Kim JE

PA5-21594 was used in Immunocytochemistry to establish the inhibition of Mps1 kinase using TC Mps1 12 as a therapeutic treatment for liver cancer patients.

Thu Jun 01 00:00:00 UTC 2017

Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells.

Cancer letters

Choi M,Kim W,Cheon MG,Lee CW,Kim JE

PA5-21594 was used in immunocytochemistry to elucidate the mechanism by which BI2536 induces defects in cell proliferation of non-small cell lung cancer

Sat Feb 28 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: HsMAD2; MAD2 (mitotic arrest deficient, homolog)-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MadL1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A

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Gene Aliases: HSMAD2; MAD2; MAD2L1

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UniProt ID: (Human) Q13257

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Entrez Gene ID: (Human) 4085, (Rat) 297176

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Function(s)

protein binding identical protein binding protein homodimerization activity

Process(es)

mitotic sister chromatid segregation mitotic cell cycle checkpoint mitotic spindle assembly checkpoint negative regulation of protein catabolic process negative regulation of apoptotic process negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle spindle assembly checkpoint negative regulation of ubiquitin protein ligase activity sister chromatid cohesion anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAD2 Polyclonal Antibody

MAD2 Antibody (PA5-21594) in ICC/IF

Product Details

PA5-21594

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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