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MAFK Antibody (MA5-24120) in WB
Western blot analysis of MAFK in A549 human lung carcinoma cell line. Samples were incubated in MAFK monoclonal antibody (Product # MA5-24120) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-24120
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
381923
Immunogen
E. coli-derived recombinant human MafK Thr2-Ser156
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610160
Product Specific Information
In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic-leucine zipper transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit; FLJ32205; MGC71717; nuclear factor erythroid-2, ubiquitous (p18); nuclear factor, erythroid derived 2, ubiquitous; p18 NF-E2; Transcription factor MafK; v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K
Gene Aliases: AW061068; MAFK; NF-E2; NFE2U; P18
UniProt ID: (Human) O60675, (Mouse) Q61827
Entrez Gene ID: (Human) 7975, (Mouse) 17135
transcription regulatory region sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RING-like zinc finger domain binding
DNA binding
protein binding
sequence-specific DNA binding
Performance Guarantee
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