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Invitrogen
MAGEE1 Polyclonal Antibody
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FIGURE: 1 / 3
MAGEE1 Antibody (PA5-103463) in ICC/IF
Immunocytochemistry analysis of MAGEE1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MAGEE1 polyclonal antibody (Product # PA5-103463) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103463
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAGEE1(Accession Q9HCI5), corresponding to amino acid residues E716-I766.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852799
Product Specific Information
Antibody detects endogenous levels of total MAGE1.
Target Information
This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein, which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus, 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT, and two MAGE domains in the C-terminus. It may play a signaling role in brain, muscle, and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to cognitive disability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-dystrobrevin-associated MAGE Protein; DAMAGE; dystrobrevin-associated MAGE protein; hepatocellular carcinoma-associated HCA1; Hepatocellular carcinoma-associated protein 1; hepatocellular carcinoma-associated protein HCA1; MAGE-E1 antigen; melanoma antigen family E, 1; melanoma antigen family E1; Melanoma-associated antigen E1
Gene Aliases: AI847422; DAMAGE; HCA1; KIAA1587; MAGEE1; mMage-e1; RGD1560259
UniProt ID: (Human) Q9HCI5, (Mouse) Q6PCZ4, (Rat) A1A5P9
Entrez Gene ID: (Human) 57692, (Mouse) 107528, (Rat) 317232
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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