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MAP2 Antibody (MA1-34378) in ICC/IF
Immunofluorescence analysis of Microtubule-associated protein 2 was performed using 70% confluent log phase SH-SY5Y cells differentiated to neurons (Retinoic acid 10 uM for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Monoclonal Antibody (MT-01) (Product # MA1-34378) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti... View More
Product Details
MA1-34378
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Cat,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MT-01
Immunogen
Termostabile fraction of bovine brain microtubules (whole MAP2ab molecule).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1958354
Product Specific Information
MA1-34378 detects MAP2 from bovine, porcine, feline, mouse samples.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; microtubule associated protein 2C; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143
Gene Aliases: BOS_2052; G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4
UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146
Entrez Gene ID: (Human) 4133, (Bovine) 281294, (Mouse) 17756, (Rat) 25595
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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