OriGene

MAP2K1 Monoclonal Antibody (OTI2B2), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF505745

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI2B2

Immunogen

Full length human recombinant protein of human MAP2K1 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dual specificity mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MEK2; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1

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UniProt ID: (Human) Q02750, (Mouse) P31938, (Rat) Q01986

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Entrez Gene ID: (Dog) 478347, (Human) 5604, (Mouse) 26395, (Rat) 170851

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding protein kinase binding protein serine/threonine kinase activator activity protein N-terminus binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding mitogen-activated protein kinase kinase kinase binding
Process(es)
MAPK cascade activation of MAPK activity regulation of protein phosphorylation regulation of vascular smooth muscle contraction protein phosphorylation response to oxidative stress cell cycle arrest mitotic nuclear division cell proliferation negative regulation of cell proliferation positive regulation of gene expression phosphorylation neuron differentiation keratinocyte differentiation positive regulation of cell migration melanosome transport regulation of stress-activated MAPK cascade positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of homotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation positive regulation of GTPase activity positive regulation of cell differentiation positive regulation of Ras protein signal transduction vesicle transport along microtubule Golgi inheritance response to axon injury regulation of axon regeneration neuron projection morphogenesis cell motility positive regulation of axonogenesis protein heterooligomerization response to glucocorticoid lung morphogenesis trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization regulation of early endosome to late endosome transport peptidyl-tyrosine phosphorylation intracellular signal transduction movement of cell or subcellular component chemotaxis signal transduction heart development negative regulation of gene expression cerebellar cortex formation thyroid gland development proteolysis in other organism positive regulation of transcription, DNA-templated thymus development Bergmann glial cell differentiation face development positive regulation of ERK1 and ERK2 cascade positive regulation of production of miRNAs involved in gene silencing by miRNA
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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