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Product Details
MAP3K8-FITC
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide taken within amino acid region 360-467 on human MAP3K8 protein.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5-1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MAP3K8 (COT) is a serine/threonine kinase which can activate both the MAP and JNK kinase pathways and plays a role in the cell cycle. Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: augmented in rheumatoid arthritis 2; Cancer Osaka thyroid oncogene; cancer Osaka thyroid, oncogene; CCOT; cot (cancer Osaka thyroid) oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 1.14.16.2; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K 8; mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Protein kinase cot; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; serine/threonine kinase (Tpl-2); Serine/threonine-protein kinase cot; TH isoform 3; TH isoform a; TH-4; TPL 2; TPL-2; Tumor progression locus 2; TY3H; TYH
Gene Aliases: AURA2; c-COT; COT; Cot/Tpl2; D17TPL2; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
UniProt ID: (Human) P41279, (Mouse) Q07174, (Rat) Q63562
Entrez Gene ID: (Human) 1326, (Mouse) 26410, (Rat) 116596
magnesium ion binding
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
immune system process
protein phosphorylation
cell cycle
signal transduction
regulation of mitotic cell cycle
phosphorylation
signal transduction by protein phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
MAPK cascade
activation of MAPKK activity
apoptotic process
intracellular signal transduction
regulation of cell differentiation
T cell costimulation
stress-activated MAPK cascade
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