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MAP4K3 Antibody (PA5-121091) in WB
Western blot analysis of MAP4K3 in extracts of various cell lines. Samples were incubated with MAP4K3 Polyclonal antibody (Product # PA5-121091) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121091
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 755-894 of human MAP4K3 (NP_003609.2).
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914663
Product Specific Information
Positive test controls include: SW480, MCF7, 293T, HepG2, Mouse brain, Mouse kidney, Mouse lung, Rat heart, Rat brain.
Immunogen sequence: ETVNPNSTSS WFTESDTPQT NVTHVTQLER DTILVCLDCC IKIVNLQGRL KSSRKLSSEL TFDFQIESIV CLQDSVLAFW KHGMQGRSFR SNEVTQEISD STRIFRLLGS DRVVVLESRP TDNPTANSNL YILAGHENSY
Target Information
MAP4K3 (also known as GLK) is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. MAP4K3 is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-alpha and can lead to the activation of the JNK signaling pathway. MAP4K3 can directly activate PKC-T, during TCR signaling and people with systemic lupus erythematosus show considerable enhanced MAP4K3 expression and activation of PKC-T, and the kinase IKK in T cells. MAP4K3 can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase 1 (MEKK1). MAP4K3 previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase 3 (MAP4K3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: germinal center kinase-like kinase; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; mitogen activated protein kinase kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase kinase 3
Gene Aliases: 4833416M01Rik; 4833416M07Rik; 9530052P13Rik; GLK; MAP4K3; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
UniProt ID: (Human) Q8IVH8, (Mouse) Q99JP0, (Rat) Q924I2
Entrez Gene ID: (Human) 8491, (Mouse) 225028, (Rat) 170920
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
ATP binding
MAP kinase kinase kinase kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
MAPK cascade
activation of MAPKKK activity
protein phosphorylation
regulation of mitotic cell cycle
response to UV
phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
response to tumor necrosis factor
intracellular signal transduction
regulation of apoptotic process
JNK cascade
signal transduction by protein phosphorylation
regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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