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MAPKAP Kinase 2 Antibody (600-401-281) in WB
Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 µg of whole cell lysate containing MK2. This antibody detects a single 44 kDa protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. A... View More
Product Details
600-401-281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish. Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
Target Information
MAPKAPK2 mediates p38 and ERK signaling in vivo. It is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation, and cell proliferation. MAPKAPK2 encodes a member of the Ser/Thr protein kinase family. MAPKAPK2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for MAPKAPK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: map kinase activated protein kinase-2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAPK-2; mitogen-activated protein kinase-activated protein kinase 2
Gene Aliases: AA960234; MAPKAP-K2; MAPKAPK2; MK-2; MK2; Rps6kc1
UniProt ID: (Human) P49137, (Mouse) P49138
Entrez Gene ID: (Rabbit) 100009438, (Human) 9261, (Mouse) 17164, (Rat) 289014
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
signal transducer activity
protein binding
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
mitogen-activated protein kinase binding
MAPK cascade
activation of MAPK activity
toll-like receptor signaling pathway
protein phosphorylation
leukotriene metabolic process
response to stress
inflammatory response
cellular response to DNA damage stimulus
Ras protein signal transduction
peptidyl-serine phosphorylation
G2 DNA damage checkpoint
response to lipopolysaccharide
regulation of interleukin-6 production
regulation of tumor necrosis factor production
response to cytokine
cellular response to vascular endothelial growth factor stimulus
p38MAPK cascade
positive regulation of tumor necrosis factor biosynthetic process
regulation of mRNA stability
macropinocytosis
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
inner ear development
3'-UTR-mediated mRNA stabilization
regulation of cellular response to heat
phosphorylation
mRNA stabilization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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