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MARK1 Polyclonal Antibody

MARK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MARK1 Antibody (21552-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using (Product # 21552-1-AP) (MARK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MARK1 Antibody in Immunoprecipitation (IP)

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Product Details

21552-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MARK1 Fusion Protein Ag16225 (506-611 aa encoded by BC113869)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VCERTTDRY VALQNGKDSS LTEMSVSSIS SAGSSVASAV PSARPRHQKS MSTSGHPIKV TLPTIKDGSE AYRPGTTQRV PAASPSAHSI STATPDRTRF PRGSSSR (506-611 aa encoded by BC113869 )

Target Information

MARK1 (MARK) is a serine/threonine kinase which is involved in cytoskeletal organization and biogenesis. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 is involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metformin reduces neuroinflammation and improves cognitive functions after traumatic brain injury.

Neuroscience research

DiBona VL,Shah MK,Krause KJ,Zhu W,Voglewede MM,Smith DM,Crockett DP,Zhang H

Mon Nov 01 00:00:00 UTC 2021

TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.

Nature communications

Chan TY,Egbert CM,Maxson JE,Siddiqui A,Larsen LJ,Kohler K,Balasooriya ER,Pennington KL,Tsang TM,Frey M,Soderblom EJ,Geng H,Müschen M,Forostyan TV,Free S,Mercenne G,Banks CJ,Valdoz J,Whatcott CJ,Foulks JM,Bearss DJ,O'Hare T,Huang DCS,Christensen KA,Moody J,Warner SL,Tyner JW,Andersen JL

Thu Sep 09 00:00:00 UTC 2021

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Cell communication and signaling : CCS

Ayanlaja AA,Ji G,Wang J,Gao Y,Cheng B,Kanwore K,Zhang L,Xiong Y,Kambey PA,Gao D

Wed Feb 12 00:00:00 UTC 2020

MicroRNA-23a promotes colorectal cancer cell migration and proliferation by targeting at MARK1.

Acta biochimica et biophysica Sinica

Tang X,Yang M,Wang Z,Wu X,Wang D

Wed Jul 10 00:00:00 UTC 2019

Loss of Par1b/MARK2 primes microglia during brain development and enhances their sensitivity to injury.

Journal of neuroinflammation

DiBona VL,Zhu W,Shah MK,Rafalia A,Ben Cheikh H,Crockett DP,Zhang H

Thu Jan 17 00:00:00 UTC 2019

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

The Journal of biological chemistry

Wu Q,Sun M,Bernard LP,Zhang H

Fri Sep 29 00:00:00 UTC 2017

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

The Journal of biological chemistry

Wu Q,Sun M,Bernard LP,Zhang H

Fri Sep 29 00:00:00 UTC 2017

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

The Journal of biological chemistry

Wu Q,Sun M,Bernard LP,Zhang H

Fri Sep 29 00:00:00 UTC 2017

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

The Journal of biological chemistry

Wu Q,Sun M,Bernard LP,Zhang H

Fri Sep 29 00:00:00 UTC 2017

DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.

Cell reports

Kwan V,Meka DP,White SH,Hung CL,Holzapfel NT,Walker S,Murtaza N,Unda BK,Schwanke B,Yuen RKC,Habing K,Milsom C,Hope KJ,Truant R,Scherer SW,Calderon de Anda F,Singh KK

Tue Nov 08 00:00:00 UTC 2016

DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.

Cell reports

Kwan V,Meka DP,White SH,Hung CL,Holzapfel NT,Walker S,Murtaza N,Unda BK,Schwanke B,Yuen RKC,Habing K,Milsom C,Hope KJ,Truant R,Scherer SW,Calderon de Anda F,Singh KK

Tue Nov 08 00:00:00 UTC 2016

MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

PloS one

Bernard LP,Zhang H

Wed Jan 27 00:00:00 UTC 2016

MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

PloS one

Bernard LP,Zhang H

Wed Jan 27 00:00:00 UTC 2016

An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential.

Molecular cell

Goodwin JM,Svensson RU,Lou HJ,Winslow MM,Turk BE,Shaw RJ

Thu Aug 07 00:00:00 UTC 2014

An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential.

Molecular cell

Goodwin JM,Svensson RU,Lou HJ,Winslow MM,Turk BE,Shaw RJ

Thu Aug 07 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: ELKL motif serine-threonine protein kinase 3; ELKL motif serine/threonine-protein kinase 3; MAP/microtubule affinity-regulating kinase 1; MARK1; mark1 {ECO:0000312|RGD:619882}; Par-1c; PAR1 homolog c; Serine/threonine-protein kinase MARK1

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Gene Aliases: AW491150; B930025N23Rik; Emk3; KIAA1477; MARK; MARK1; mKIAA1477; mPar-1c; Par-1c; Par1c

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UniProt ID: (Human) Q9P0L2, (Mouse) Q8VHJ5, (Rat) O08678

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Entrez Gene ID: (Human) 4139, (Mouse) 226778, (Rat) 117016

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Function(s)

magnesium ion binding phosphatidylserine binding protein serine/threonine kinase activity ATP binding phosphatidylinositol-4,5-bisphosphate binding tau-protein kinase activity phosphatidic acid binding nucleotide binding protein kinase activity lipid binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding

Process(es)

microtubule cytoskeleton organization neuron migration protein phosphorylation cytoskeleton organization Wnt signaling pathway establishment of cell polarity intracellular signal transduction phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MARK1 Polyclonal Antibody

MARK1 Antibody (21552-1-AP) in IHC (P)

Product Details

21552-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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