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          • Primary Antibodies ›
          • MARVELD2 Antibodies

          Invitrogen

          MARVELD2 Polyclonal Antibody

          20 Published Figures
          26 References
          View all (7) MARVELD2 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite MARVELD2 Polyclonal Antibody

          • Antibody Testing Data (5)
          • Published Figures (20)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 25

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          MARVELD2 Antibody (48-8400) in ICC/IF

          Immunofluorescence analysis of Marveld2/ Tricellulin was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MARVELD2/Tricellulin Rabbit Polyclonal Antibody (Product # 48-8400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fl... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Immunocytochemistry (ICC/IF)
          MARVELD2 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          MARVELD2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          MARVELD2 Antibody in Immunohistochemistry (IHC)
          MARVELD2 Antibody in Immunohistochemistry (IHC)
          MARVELD2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)
          MARVELD2 Antibody in Western Blot (WB)

          Product Details

          48-8400

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-3 µg/mL
          View 14 publications 14 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2-4 µg/mL
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          3-5 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Dog, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein derived from the C-terminal region of the human Tricellulin protein (Accession# NP_001033692, Q8N4S9), which is identical to rhesus monkey and chimpanzee sequences, and 89% homologous to rat and mouse, 88% homologous to bovine and canine, and 86% homologous to horse sequence
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533851

          Product Specific Information

          This antibody is specific for the Tricellulin/MARVELD-2 protein. On western blots, it identifies the target band at ~64 kDa. Reactivity has been confirmed with human Caco-2, dog MDCK, mouse IMCD-3 cells, and mouse kidney lysates by western blotting, and with mouse MTE7b cells by immunocytochemistry. Based on amino acid sequence homology, reactivity with Rhesus monkey, chimpanzee, rat, bovine, and horse is expected.

          Target Information

          Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions, a point where three adjacent cells are in contact with each other. Tricellulin is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane spanning domains, similarly to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Loss of function in tricellulin mutants missing all or most of a conserved region in the cytosolic domain which binds to the cytosolic scaffolding protein ZO-1, indicate that interaction with other known tight junction proteins plays an important role for the function of tricellulin.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: MARVEL (membrane-associating) domain containing 2; MARVEL domain-containing protein 2; Tricellulin

          View more View less

          Gene Aliases: BC003296; DFNB49; MARVD2; MARVELD2; MRVLDC2; TRIC; Trica; Tricb; Tricc

          View more View less

          UniProt ID: (Human) Q8N4S9, (Mouse) Q3UZP0

          View more View less

          Entrez Gene ID: (Human) 153562, (Mouse) 218518, (Rat) 365657

          View more View less

          Function(s)
          protein binding molecular_function
          Process(es)
          sensory perception of sound cell-cell junction organization establishment of endothelial barrier bicellular tight junction assembly
          It has to be done as per old AB suggested Products section.
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