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MARVELD2 Antibody (PA5-106999) in WB
Western blot was performed using Anti-MARVELD2 Polyclonal Antibody (Product # PA5-106999) and 64, 62 kDa bands corresponding to MARVEL domain-containing protein 2 were observed across tested cell lines along with uncharacterized (*) band at ~30 kDa. Membrane enriched extracts (40 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), A-431 (Lane 5), HaCaT (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose memb... View More
Product Details
PA5-106999
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the middle region of human MARVELD2 (aa 251-301)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.4, with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2817715
Product Specific Information
Sequence homology: Cow: 100%; Dog: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
Target Information
Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions, a point where three adjacent cells are in contact with each other. Tricellulin is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane spanning domains, similarly to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Loss of function in tricellulin mutants missing all or most of a conserved region in the cytosolic domain which binds to the cytosolic scaffolding protein ZO-1, indicate that interaction with other known tight junction proteins plays an important role for the function of tricellulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MARVEL (membrane-associating) domain containing 2; MARVEL domain-containing protein 2; Tricellulin
Gene Aliases: DFNB49; MARVD2; MARVELD2; MRVLDC2; TRIC
UniProt ID: (Human) Q8N4S9
Entrez Gene ID: (Human) 153562
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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