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Invitrogen
MASH1 Monoclonal Antibody (24B72D11), eBioscience™
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Product Details
14-5794-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
24B72D11
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572887
Product Specific Information
Description: The monoclonal antibody 24B72D11 recognizes mouse and rat Mammalian achaete-scute homolog 1 (MASH1), a 34 kDa basic helix-loop-helix (bHLH) transcription factor. MASH1, like family members Olig2 and Ngn2, is expressed in progenitor cells throughout the nervous system and retina and is a key regulator of cell fate specification. Expression of MASH1 is regulated by the Delta/Notch1 pathway through direct binding of Hes1 to MASH1 as well as competitive binding to MASH1 hetero-dimerizing proteins. At a transcriptional level, MASH1 expression is controlled by notch-mediated regulation of sox2, which binds to the MASH1 promoter region up-regulating expression. MASH1 has been implicated in the formation of multiple neural cell types including dopaminergic, serotonergic, glutaminergic and GABAergic neurons. Overexpression of MASH1 has been shown in small cell lung carcinoma and medullary thyroid cancer.
Applications Reported: This 24B72D11 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This 24B72D11 antibody has been tested by western blot on reduced PC12 cells and can be used at less than or equal to 5 µg/mL. The 24B72D11 antibody has also been tested by immunocytochemistry on fixed and permeablized PC12 cells at can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; KMT2H; mammalian achaete scute homolog 1; mASH-1
Gene Aliases: AI225900; Ascl1; ASH1; bHLHa46; Mash-1; Mash1
UniProt ID: (Rat) P19359, (Mouse) Q02067
Entrez Gene ID: (Rat) 64186, (Mouse) 17172
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
bHLH transcription factor binding
sequence-specific DNA binding
E-box binding
protein binding
protein dimerization activity
negative regulation of transcription from RNA polymerase II promoter
neuron migration
noradrenergic neuron development
noradrenergic neuron fate commitment
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
Notch signaling pathway
regulation of mitotic cell cycle
pattern specification process
nervous system development
neuroblast fate determination
neuroblast proliferation
regulation of Notch signaling pathway
glial cell differentiation
response to lithium ion
regulation of gene expression
oligodendrocyte development
spinal cord association neuron differentiation
spinal cord oligodendrocyte cell differentiation
spinal cord oligodendrocyte cell fate specification
vestibular nucleus development
oligodendrocyte cell fate commitment
forebrain neuron differentiation
cerebral cortex GABAergic interneuron differentiation
commitment of neuronal cell to specific neuron type in forebrain
central nervous system neuron development
cerebral cortex development
neurogenesis
cell differentiation
neuron differentiation
regulation of epithelial cell differentiation
response to retinoic acid
regulation of cell proliferation
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of Notch signaling pathway
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell maturation
neuron fate commitment
neuron fate specification
neuron development
generation of neurons
oligodendrocyte differentiation
regulation of neurogenesis
positive regulation of neurogenesis
musculoskeletal movement, spinal reflex action
response to folic acid
subpallium neuron fate commitment
regulation of timing of subpallium neuron differentiation
olfactory pit development
ventral spinal cord interneuron fate commitment
lung neuroendocrine cell differentiation
stomach neuroendocrine cell differentiation
carotid body glomus cell differentiation
adrenal chromaffin cell differentiation
sympathetic ganglion development
response to epidermal growth factor
cellular response to magnetism
positive regulation of neural precursor cell proliferation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
enteric nervous system development
sympathetic nervous system development
parasympathetic nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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