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Invitrogen
MBD2 Recombinant Rabbit Monoclonal Antibody (4D4D5)
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FIGURE: 1 / 1
MBD2 Antibody (MA5-42844) in WB
Western blot analysis of MBD2 in extracts of Mouse liver cells. Samples were incubated with MBD2 Monoclonal antibody (Product # MA5-42844) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-42844
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4D4D5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MBD2 (Q9UBB5).
Conjugate
Form
Liquid
Concentration
2.18 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911985
Product Specific Information
Positive test controls include: Mouse liver.
Immunogen sequence: GGSGLGGDGG GCGGGGSGGG GAPRREPVPF PSGSAGPGPR GPRATESGKR MDCPALPPGW KKEEVIRKSG LSAGKSDVYY FSPSGKKFRS KPQLARYLGN T
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586O0821; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2
Gene Aliases: Mbd2; MBD2a
UniProt ID: (Mouse) Q9Z2E1
Entrez Gene ID: (Mouse) 17191
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
DNA binding
chromatin binding
mRNA binding
protein binding
methyl-CpG binding
protein domain specific binding
nucleosomal DNA binding
siRNA binding
C2H2 zinc finger domain binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of Wnt signaling pathway
positive regulation of chromatin binding
regulation of cell proliferation
maternal behavior
ATP-dependent chromatin remodeling
cellular protein complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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