Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
MBD3 Recombinant Rabbit Monoclonal Antibody (JB61-47)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 12
MBD3 Antibody (MA5-34711) in ICC/IF
Immunofluorescence analysis of Methyl-CpG-binding protein MBD3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MBD3 Recombinant Rabbit Monoclonal Antibody (JB61-47) (Product # MA5-34711) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Se... View More
Product Details
MA5-34711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB61-47
Immunogen
Recombinant protein within Human MBD3 aa 160-291
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848619
Product Specific Information
Positive Control: U937, A549, NIH-3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue.
Target Information
Regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. Necessary for centrosome duplication. It functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CpG binding domain 3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
Gene Aliases: AI181826; AU019209; MBD3
UniProt ID: (Human) O95983, (Mouse) Q9Z2D8
Entrez Gene ID: (Human) 53615, (Mouse) 17192
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
methylation-dependent chromatin silencing
transcription, DNA-templated
brain development
heart development
aging
tissue development
histone acetylation
histone deacetylation
response to nutrient levels
response to estradiol
ATP-dependent chromatin remodeling
regulation of DNA methylation
embryonic organ development
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support