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MBL2 Antibody (PA5-79640) in IHC (P)
Immunohistochemistry analysis of MBL2 on paraffin-embedded rat liver tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with MBL2 polyclonal antibody (Product# PA5-79640) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development ... View More
Product Details
PA5-79640
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat MBL2 recombinant protein (Position: E19-D244).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746755
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse liver tissue, mouse HEPA1-6 whole cell. IHC: mouse liver tissue.
Target Information
MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collectin1; Mannan-binding protein; mannose binding lectin (C); mannose binding lectin 2 (protein C); mannose binding lectin, liver (C); Mannose-binding protein C; mannose-binding protein C (liver); MBP-C; MBPC; MGC116832; MGC116833; RA-reactive factor P28A subunit; Ra-reactive factor polysaccharide-binding component p28A; RaRF p28A; RARF/P28A
Gene Aliases: Ab2-001; Ab2-011; L-MBP; MBL; MBL-C; Mbl2; MBP-C
UniProt ID: (Mouse) P41317, (Rat) P08661
Entrez Gene ID: (Mouse) 17195, (Rat) 64668
complement activation, lectin pathway
immune system process
complement activation, classical pathway
negative regulation of growth of symbiont in host
innate immune response
negative regulation of viral process
positive regulation of phagocytosis
defense response to Gram-positive bacterium
killing by host of symbiont cells
positive regulation of protein processing
positive regulation of complement activation
protein oligomerization
protein homooligomerization
protein homotrimerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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