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MC3R Antibody (PA5-88171) in WB
Western blot analysis of MC3R in extracts of various cell lines. Samples were incubated with MC3R Polyclonal antibody (Product # PA5-88171) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88171
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human MC3R (NP_0639413)
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804713
Product Specific Information
Immunogen sequence: MNASCCLPSV QPTLPNGSEH LQAPFFSNQS SSAFCEQVFI KPEVFLSLGI VSLLENILVI LAVVRNGNLH SPMYFFLCSL AVADMLVSVS NALETIMIAI VHSDYLTFED QFIQHMDNIF; Positive Samples: Mouse brain, Mouse liver, Mouse kidney, Rat brain; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
This gene encodes a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis. Mutations in this gene are associated with a susceptibility to obesity in humans.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MC3 receptor; MC3-R; Melanocortin receptor 3
Gene Aliases: MC3-R; Mc3r
UniProt ID: (Mouse) P33033, (Rat) P32244
Entrez Gene ID: (Mouse) 17201, (Rat) 29310
regulation of heart rate
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
regulation of blood pressure
positive regulation of cAMP biosynthetic process
circadian regulation of gene expression
homoiothermy
locomotor rhythm
rhythmic process
regulation of feeding behavior
sodium ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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