Proteintech

MCC Polyclonal Antibody

View all (10) MCC antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (8)

MCC Antibody in Immunocytochemistry (ICC/IF)

MCC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

MCC Antibody (11525-1-AP) in ICC/IF

Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCC Antibody in Immunocytochemistry (ICC/IF)

MCC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MCC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MCC Antibody in Western Blot (WB)

MCC Antibody in Western Blot (WB)

MCC Antibody in Western Blot (WB)

MCC Antibody in Western Blot (WB)

MCC Antibody in Immunoprecipitation (IP)

Product Image Gallery

MCC Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue slide using (Product # 11525-1-AP) MCC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MCC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 11525-1-AP (MCC antibody) at dilution of 1:100 (under 10x lens).

MCC Antibody in Western Blot (WB) — Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP ( MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCC Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MCC Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MCC Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCC Antibody in Immunoprecipitation (IP) — IP result of anti-MCC (IP:11525-1-AP, 3ug; Detection:11525-1-AP 1:500) with PC-3 cells lysate 5000ug.

Product Details

11525-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCC Fusion Protein Ag2089 (480-829 aa encoded by BC018919)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AGVGSSPGD QSGDENITQM LKRAHDCRKT AENAAKALLM KLDGSCGGAF AVAGCSVQPW ESLSSNSHTS TTSSTASSCD TEFTKEDEQR LKDYIQQLKN DRAAVKLTML ELESIHIDPL SYDVKPRGDS QRLDLENAVL MQELMAMKEE MAELKAQLYL LEKEKKALEL KLSTREAQEQ AYLVHIEHLK SEVEEQKEQR MRSLSSTSSG SKDKPGKECA DAASPALSLA ELRTTCSENE LAAEFTNAIR REKKLKARVQ ELVSALERLT KSSEIRHQQS AEFVNDLKRA NSNLVAAYEK AKKKHQNKLK KLESQMMAMV ERHETQVRML KQRIALLEEE NSRPHTNETS L (480-829 aa encoded by BC018919)

Target Information

This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Frontiers in medicine

Song H,Zhuang L,Xu X,Shi J,Hu W,Liu Z,Shi S

Tue Dec 21 00:00:00 UTC 2021

'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.

Oncogene

Benthani FA,Herrmann D,Tran PN,Pangon L,Lucas MC,Allam AH,Currey N,Al-Sohaily S,Giry-Laterriere M,Warusavitarne J,Timpson P,Kohonen-Corish MRJ

Thu Feb 01 00:00:00 UTC 2018

'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.

Oncogene

Benthani FA,Herrmann D,Tran PN,Pangon L,Lucas MC,Allam AH,Currey N,Al-Sohaily S,Giry-Laterriere M,Warusavitarne J,Timpson P,Kohonen-Corish MRJ

Thu Feb 01 00:00:00 UTC 2018

Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.

Proteomics

Corbo C,Orrù S,Gemei M,Noto RD,Mirabelli P,Imperlini E,Ruoppolo M,Vecchio LD,Salvatore F

Fri Jun 01 00:00:00 UTC 2012

Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.

Proteomics

Corbo C,Orrù S,Gemei M,Noto RD,Mirabelli P,Imperlini E,Ruoppolo M,Vecchio LD,Salvatore F

Fri Jun 01 00:00:00 UTC 2012

Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.

PloS one

Rohrbeck A,Borlak J

Thu Oct 08 00:00:00 UTC 2009

Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.

PloS one

Rohrbeck A,Borlak J

Thu Oct 08 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Colorectal mutant cancer protein; Protein MCC

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Gene Aliases: D18Ertd451e; E330037C19; MCC; MCC1

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UniProt ID: (Human) P23508

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Entrez Gene ID: (Human) 4163, (Mouse) 328949

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receptor activity protein binding

negative regulation of epithelial cell migration establishment of protein localization negative regulation of epithelial cell proliferation negative regulation of canonical Wnt signaling pathway signal transduction Wnt signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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