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MCHR2 Antibody (PA5-102101) in ICC/IF
Immunocytochemistry analysis of MCHR2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MCHR2 polyclonal antibody (Product # PA5-102101) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MCHR2(Accession Q969V1), corresponding to amino acid residues S299-F340.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851528
Product Specific Information
Antibody detects endogenous levels of total MCHR2.
Target Information
Melanin-concentrating hormone (MCH) is a 19 amino acid cyclic neuropeptide which is mainly expressed in the hypothalamus. MCH modulates feeding behavior, aggression, anxiety, arousal and reproductive function in mammals by controlling the release of luteinizing hormone (LH). The melanin-concentrating hormone receptor (MCHR, also designated SLC-1) is a glycosylated G-protein coupled receptor that migrates between 45-50 kDa on SDS-PAGE. MCHR mediates the effects of MCH through Gai and/or Gaq signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus and hypothalamus. MCH and MCHR have also been implicated in stimulating leptin expression and secretion in adipocytes, which suggests that the melanin-concentrating hormone and its receptor may be potential targets for modulating obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 145; G protein-coupled receptor slt; G-protein coupled receptor 145; GPRv17; MCH receptor 2; MCH-2R; MCH2; Mch2 receptor; melanin-concentrating hormone 2; Melanin-concentrating hormone receptor 2; SLT receptor
Gene Aliases: GPR145; GPRv17; MCH-2R; MCH-R2; MCH2; MCH2R; MCHR-2; MCHR2; SLT
UniProt ID: (Human) Q969V1
Entrez Gene ID: (Human) 84539
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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