Proteintech

MCM10 Polyclonal Antibody

MCM10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

MCM10 Antibody (12251-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12251-1-AP (MCM10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Product Image Gallery

Product Details

12251-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCM10 Fusion Protein Ag2899 (259-611 aa encoded by BC009108)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDLPTCGAR NLKQHLAKAS ASGIMGSPKP AIKSISASAL LKQQKQRMLE MRRRKSEEIQ KRFLQSSSEV ESPAVPSSSR QPPAQPPRTG SEFPRLEGAP ATMTPKLGRG VLEGDDVLFY DESPPPRPKL SALAEAKKLA AITKLRAKGQ VLTKTNPNSI KKKQKDPQDI LEVKERVEKN TMFSSQAEDE LEPARKKRRE QLAYLESEEF QKILKAKSKH TGILKEAEAE MQERYFEPLV KKEQMEEKMR NIREVKCRVV TCKTCAYTHF KLLETCVSEQ HEYHWHDGVK RFFKCPCGNR SISLDRLPNK HCSNCGLYKW ERDGMLKEKT GPKIGGETLL PRGEEHAKFL NSLK (259-611 aa encoded by B C009108)

Target Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of Novel Hub Genes Associated with Psoriasis Using Integrated Bioinformatics Analysis.

International journal of molecular sciences

Yue Q,Li Z,Zhang Q,Jin Q,Zhang X,Jin G

Sun Dec 04 00:00:00 UTC 2022

Identification of Candidate Genes in Breast Cancer Induced by Estrogen Plus Progestogens Using Bioinformatic Analysis.

International journal of molecular sciences

Deng Y,Huang H,Shi J,Jin H

Thu Oct 06 00:00:00 UTC 2022

BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.

Nature communications

Kang Z,Fu P,Alcivar AL,Fu H,Redon C,Foo TK,Zuo Y,Ye C,Baxley R,Madireddy A,Buisson R,Bielinsky AK,Zou L,Shen Z,Aladjem MI,Xia B

Wed Oct 13 00:00:00 UTC 2021

Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins.

Viruses

Chang H,Siarot L,Matsuura R,Lo CW,Sato H,Otsuki H,Aida Y

Wed Jan 15 00:00:00 UTC 2020

Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins.

Viruses

Chang H,Siarot L,Matsuura R,Lo CW,Sato H,Otsuki H,Aida Y

Wed Jan 15 00:00:00 UTC 2020

Constructing Auxin-Inducible Degron Mutants Using an All-in-One Vector.

Pharmaceuticals (Basel, Switzerland)

Yesbolatova A,Saito Y,Kanemaki MT

Sat May 23 00:00:00 UTC 2020

DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.

Nature communications

Zhang J,Bellani MA,James RC,Pokharel D,Zhang Y,Reynolds JJ,McNee GS,Jackson AP,Stewart GS,Seidman MM

Fri Aug 07 00:00:00 UTC 2020

Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.

The Journal of cell biology

Achuthankutty D,Thakur RS,Haahr P,Hoffmann S,Drainas AP,Bizard AH,Weischenfeldt J,Hickson ID,Mailand N

Mon Dec 02 00:00:00 UTC 2019

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 UTC 2017

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 UTC 2017

TIMELESS Suppresses the Accumulation of Aberrant CDC45·MCM2-7·GINS Replicative Helicase Complexes on Human Chromatin.

The Journal of biological chemistry

Xu X,Wang JT,Li M,Liu Y

Fri Oct 21 00:00:00 UTC 2016

TIMELESS Suppresses the Accumulation of Aberrant CDC45·MCM2-7·GINS Replicative Helicase Complexes on Human Chromatin.

The Journal of biological chemistry

Xu X,Wang JT,Li M,Liu Y

Fri Oct 21 00:00:00 UTC 2016

Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction.

Cell reports

Wang JT,Xu X,Alontaga AY,Chen Y,Liu Y

Thu May 08 00:00:00 UTC 2014

Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction.

Cell reports

Wang JT,Xu X,Alontaga AY,Chen Y,Liu Y

Thu May 08 00:00:00 UTC 2014

Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination.

PloS one

Anantha RW,Alcivar AL,Ma J,Cai H,Simhadri S,Ule J,König J,Xia B

Tue Oct 22 00:00:00 UTC 2013

Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination.

PloS one

Anantha RW,Alcivar AL,Ma J,Cai H,Simhadri S,Ule J,König J,Xia B

Tue Oct 22 00:00:00 UTC 2013

DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.

Cancer discovery

Mazurek A,Luo W,Krasnitz A,Hicks J,Powers RS,Stillman B

Sat Sep 01 00:00:00 UTC 2012

DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.

Cancer discovery

Mazurek A,Luo W,Krasnitz A,Hicks J,Powers RS,Stillman B

Sat Sep 01 00:00:00 UTC 2012

MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

The EMBO journal

Xu X,Rochette PJ,Feyissa EA,Su TV,Liu Y

Wed Oct 07 00:00:00 UTC 2009

MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

The EMBO journal

Xu X,Rochette PJ,Feyissa EA,Su TV,Liu Y

Wed Oct 07 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: homolog of yeast MCM10; HsMCM10; MCM10 minichromosome maintenance deficient 10; MGC126776; minichromosome maintenance complex component 10; Protein MCM10 homolog

View more View less

Gene Aliases: 2410041F14Rik; AU018508; C330019M07Rik; C79164; CNA43; DNA43; MCM10; PRO2249

View more View less

UniProt ID: (Human) Q7L590, (Mouse) Q0VBD2

View more View less

Entrez Gene ID: (Human) 55388, (Mouse) 70024

View more View less

Function(s)

DNA replication origin binding single-stranded DNA binding protein binding enzyme binding identical protein binding metal ion binding DNA binding double-stranded DNA binding

Process(es)

G1/S transition of mitotic cell cycle DNA replication DNA replication initiation cellular response to DNA damage stimulus cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MCM10 Polyclonal Antibody

MCM10 Antibody (12251-1-AP) in ICC/IF

Product Details

12251-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!