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MCM2 Antibody (600-401-922) in WB
Western blot using Rocklands Affinity Purified anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 present in nuclear extracts from elutriated human cells (MO59K/K562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the protein is unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (lane 1), cells arrested in earl... View More
Product Details
600-401-922
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 15-40 of human MCM2 protein.
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human MCM2 protein. Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27). A BLAST analysis was used to suggest cross reactivity with MCM2 proteins from human, mouse, rat and S. cerevisiae based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; Mcm2p; MGC10606; mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Gene Aliases: AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; Mcmd2; MITOTIN; mKIAA0030; YBL023C; YBL0438
UniProt ID: (Human) P49736, (Baker's yeast) P29469, (Mouse) P97310
Entrez Gene ID: (Human) 4171, (Baker's yeast) 852258, (Rat) 312538, (Mouse) 17216
G1/S transition of mitotic cell cycle
DNA replication
DNA unwinding involved in DNA replication
DNA replication initiation
nucleosome assembly
cell cycle
cellular response to interleukin-4
mitotic S phase
double-strand break repair via break-induced replication
pre-replicative complex assembly involved in nuclear cell cycle DNA replication
DNA strand elongation involved in DNA replication
cellular response to DNA damage stimulus
DNA duplex unwinding
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