Proteintech

MCM4 Polyclonal Antibody

FIGURE: 1 / 5

MCM4 Antibody (13043-1-AP) in WB

Human liver tissue were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MCM4 Antibody in Immunoprecipitation (IP)

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Product Details

13043-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCM4 Fusion Protein Ag3703 (515-859 aa encoded by BC031061)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein.

Immunogen sequence: SKSQLLQYV YNLVPRGQYT SGKGSSAVGL TAYVMKDPET RQLVLQTGAL VLSDNGICCI DEFDKMNEST RSVLHEVMEQ QTLSIAKAGI ICQLNARTSV LAAANPIESQ WNPKKTTIEN IQLPHTLLSR FDLIFLMLDP QDEAYDRRLA HHLVALYYQS EEQAEEELLD MAVLKDYIAY AHSTIMPRLS EEASQALIEA YVDMRKIGSS RGMVSAYPRQ LESLIRLAEA HAKVRLSNKV EAIDVEEAKR LHREALKQSA TDPRTGIVDI SILTTGMSAT SRKRKEELAE ALKKLILSKG KTPALKYQQL FEDIRGQSDI AITKDMFEEA LRALADDDFL TVTGKT (515-859 aa encoded by BC031061)

Target Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Biology

Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

Fri Feb 11 00:00:00 UTC 2022

ACTL6A deficiency induces apoptosis through impairing DNA replication and inhibiting the ATR-Chk1 signaling in glioblastoma cells.

Biochemical and biophysical research communications

Hu X,Pei D,Ci M,Zhang G,Li B,Wang J,Shen Y,Zhai X,Liang P,Cui H

Sat Apr 09 00:00:00 UTC 2022

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Ecotoxicology and environmental safety

Li Z,Yin H,Shen Y,Ren M,Xu X

Mon Mar 15 00:00:00 UTC 2021

S-Adenosylmethionine Affects Cell Cycle Pathways and Suppresses Proliferation in Liver Cells.

Journal of Cancer

Yan L,Liang X,Huang H,Zhang G,Liu T,Zhang J,Chen Z,Zhang Z,Chen Y

Wed Sep 30 00:00:00 UTC 2020

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 UTC 2018

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 UTC 2018

RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.

Oncotarget

Fei L,Ma Y,Zhang M,Liu X,Luo Y,Wang C,Zhang H,Zhang W,Han Y

Tue Jun 20 00:00:00 UTC 2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 UTC 2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 UTC 2017

A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly.

Nature structural & molecular biology

Eisenhardt N,Chaugule VK,Koidl S,Droescher M,Dogan E,Rettich J,Sutinen P,Imanishi SY,Hofmann K,Palvimo JJ,Pichler A

Tue Dec 01 00:00:00 UTC 2015

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.

PloS one

Valle BL,D'Souza T,Becker KG,Wood WH,Zhang Y,Wersto RP,Morin PJ

Mon Dec 09 00:00:00 UTC 2013

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.

PloS one

Valle BL,D'Souza T,Becker KG,Wood WH,Zhang Y,Wersto RP,Morin PJ

Mon Dec 09 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CDC21 homolog; DNA replication licensing factor MCM4; homolog of S. pombe cell devision cycle 21; minichromosome maintenance deficient 4; P1-CDC21

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Gene Aliases: CDC21; CDC54; hCdc21; MCM4; NKCD; NKGCD; P1-CDC21

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UniProt ID: (Human) P33991

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Entrez Gene ID: (Human) 4173

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Function(s)

single-stranded DNA binding ATP-dependent DNA helicase activity protein binding ATP binding

Process(es)

G1/S transition of mitotic cell cycle DNA replication DNA unwinding involved in DNA replication DNA replication initiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MCM4 Polyclonal Antibody

MCM4 Antibody (13043-1-AP) in WB

Product Details

13043-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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