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MCM6 Antibody (MA5-50666) in ICC/IF
Immunocytochemistry analysis of MCM6 in SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with MCM6 recombinant monoclonal antibody (Product # MA5-50666) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1:1,000. Nuclear DNA was la... View More
Product Details
MA5-50666
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE65-68
Immunogen
Recombinant protein within human MCM6 aa 572-821/821.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094152
Product Specific Information
Positive control: Hela cell lysate, Jurkat cell lysate, MCF-7 cell lysate, RAW264.7 cell lysate, SiHa, rat skin tissue, human lymph nodes tissue, human testis tissue, mouse stomach tissue.
Predicted band size: 93 kDa
Subcellular Location: Nucleus, Chromosome.
Target Information
MCM6 may be involved in the control of a single round of DNA replication during S phase. It binds to chromatin during G1 and detaches from it during S phase as if it licenses the chromatin to replicate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA replication licensing factor MCM6; intestinal DNA replication protein; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); mini chromosome maintenance deficient 6; minichromosome maintenance deficient (mis5, S. pombe) 6; minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); Mis5 homolog; p105MCM
Gene Aliases: ASP-l1; D1Wsu22e; MCG40308; MCM6; Mcmd6; Mis5; P105MCM
UniProt ID: (Human) Q14566, (Mouse) P97311
Entrez Gene ID: (Human) 4175, (Rat) 29685, (Mouse) 17219
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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