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MCM7 Antibody (A302-584A) in WB
Detection of human MCM7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MCM7 antibody (Product # A302-584A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. MCM7 was also immunoprecipitated by rabbit anti-MCM7 antibodies A302-585A and A302-586A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3... View More
Product Details
A302-584A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 300 and 350 of human minichromosome maintenance deficient 7.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; minichromosome maintenance deficient 7; P1 P85MCM; P1.1-MCM3; protein phosphatase 1, regulatory subunit 104
Gene Aliases: CDC47; MCM2; MCM7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
UniProt ID: (Human) P33993
Entrez Gene ID: (Human) 4176
G1/S transition of mitotic cell cycle
DNA replication
DNA unwinding involved in DNA replication
DNA replication initiation
cellular response to DNA damage stimulus
cell proliferation
regulation of phosphorylation
response to drug
cellular response to epidermal growth factor stimulus
cellular response to xenobiotic stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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