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Invitrogen
MCM7 Monoclonal Antibody (3H11)
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FIGURE: 1 / 10
MCM7 Antibody (MA5-49205) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of MCM7 in PC-3 cells using MCM7 Monoclonal Antibody (3H11) (Product # MA5-49205). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter... View More
Product Details
MA5-49205
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3H11
Immunogen
E.coli-derived human MCM7 recombinant protein (Position: D526-V719).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074863
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human MCM7 shares 94% amino acid (aa) sequence identity with MCM7.
Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human MDA-MB-231 whole cell, rat C6 whole cell, human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human U2OS whole cell, human A549 whole cell. IHC: human laryngeal squamous cell carcinoma tissue, human adenocarcinoma of the colon tissue, human chronic tonsillitis tissue. ICC/IF: PC-3 cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; minichromosome maintenance deficient 7; minichromosome maintenance protein 7; P1 P85MCM; P1.1-MCM3; protein phosphatase 1, regulatory subunit 104
Gene Aliases: CDC47; MCM2; MCM7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
UniProt ID: (Human) P33993
Entrez Gene ID: (Human) 4176, (Rat) 288532
G1/S transition of mitotic cell cycle
DNA replication
DNA unwinding involved in DNA replication
DNA replication initiation
cellular response to DNA damage stimulus
cell proliferation
regulation of phosphorylation
response to drug
cellular response to epidermal growth factor stimulus
cellular response to xenobiotic stimulus
regulation of transcription, DNA-templated
cellular response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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