Invitrogen

MCP-1 Polyclonal Antibody, PeproTech®

1 Published Figure

15 References

View all (67) CCL2 (MCP-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

FIGURE: 1 / 4

MCP-1 Antibody (500-P34-1MG) in WB

Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis MCP-1 Polyclonal Antibody (Product # 500-P34-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P34-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

Neutralization (Neu)

View 2 publications 2 publications

In vitro Assay (IV)

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Immunostaining (IS)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MCP-1 (CCL2)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

AB_2930474

Product Specific Information

AA Sequence of recombinant protein: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-1 (CCL2) matrix.

Sandwich ELISA: To detect Human MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-1 (CCL2) (500-P34Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-1 (CCL2).

Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Two glycosaminoglycan-binding domains of the mouse cytomegalovirus-encoded chemokine MCK-2 are critical for oligomerization of the full-length protein.

The Journal of biological chemistry

Pontejo SM,Murphy PM

Fri Jun 09 00:00:00 UTC 2017

Vitamin D [1,25(OH)2D3] Differentially Regulates Human Innate Cytokine Responses to Bacterial versus Viral Pattern Recognition Receptor Stimuli.

Journal of immunology (Baltimore, Md. : 1950)

Fitch N,Becker AB,HayGlass KT

Fri Apr 01 00:00:00 UTC 2016

Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs.

Neoplasia (New York, N.Y.)

Lebel-Haziv Y,Meshel T,Soria G,Yeheskel A,Mamon E,Ben-Baruch A

500-P34 was used in Enzyme-linked immunosorbent assay to propose that targeting these chemokine regions may lead to reduced secretion of CCL2 by breast cancer cells (and potentially also by other malignant cells).

Mon Sep 01 00:00:00 UTC 2014

Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs.

Neoplasia (New York, N.Y.)

Lebel-Haziv Y,Meshel T,Soria G,Yeheskel A,Mamon E,Ben-Baruch A

Mon Sep 01 00:00:00 UTC 2014

Activation of thromboxane A2 receptor (TP) increases the expression of monocyte chemoattractant protein -1 (MCP-1)/chemokine (C-C motif) ligand 2 (CCL2) and recruits macrophages to promote invasion of lung cancer cells.

PloS one

Li X,Tai HH

Wed Jul 24 00:00:00 UTC 2013

KRAS(G12V) enhances proliferation and initiates myelomonocytic differentiation in human stem/progenitor cells via intrinsic and extrinsic pathways.

The Journal of biological chemistry

Fatrai S,van Gosliga D,Han L,Daenen SM,Vellenga E,Schuringa JJ

Fri Feb 25 00:00:00 UTC 2011

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 UTC 2010

Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Nakazawa T,Hisatomi T,Nakazawa C,Noda K,Maruyama K,She H,Matsubara A,Miyahara S,Nakao S,Yin Y,Benowitz L,Hafezi-Moghadam A,Miller JW

Tue Feb 13 00:00:00 UTC 2007

Dexamethasone modulates interleukin-12 production by inducing monocyte chemoattractant protein-1 in human dendritic cells.

Immunology and cell biology

Roca L,Di Paolo S,Petruzzelli V,Grandaliano G,Ranieri E,Schena FP,Gesualdo L

Fri Feb 15 00:00:00 UTC 2008

Microglial cells from psychologically stressed mice as an accelerator of cerebral cryptococcosis.

Immunology and cell biology

Shimoda M,Jones VC,Kobayashi M,Suzuki F

Fri Dec 01 00:00:00 UTC 2006

MCP-1 is induced by receptor activator of nuclear factor-{kappa}B ligand, promotes human osteoclast fusion, and rescues granulocyte macrophage colony-stimulating factor suppression of osteoclast formation.

The Journal of biological chemistry

Kim MS,Day CJ,Morrison NA

Fri Apr 22 00:00:00 UTC 2005

Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages.

Blood

Fantuzzi L,Spadaro F,Vallanti G,Canini I,Ramoni C,Vicenzi E,Belardelli F,Poli G,Gessani S

Wed Oct 01 00:00:00 UTC 2003

C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs.

Journal of applied physiology (Bethesda, Md. : 1985)

Turk JR,Carroll JA,Laughlin MH,Thomas TR,Casati J,Bowles DK,Sturek M

Mon Sep 01 00:00:00 UTC 2003

The CC chemokine RANTES in breast carcinoma progression: regulation of expression and potential mechanisms of promalignant activity.

Cancer research

Azenshtein E,Luboshits G,Shina S,Neumark E,Shahbazian D,Weil M,Wigler N,Keydar I,Ben-Baruch A

Fri Feb 15 00:00:00 UTC 2002

STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.

The Journal of clinical investigation

Lentsch AB,Kato A,Davis B,Wang W,Chao C,Edwards MJ

Thu Nov 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

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Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

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UniProt ID: (Human) P13500

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Entrez Gene ID: (Human) 6347

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Function(s)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding

Process(es)

Performance Guarantee

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MCP-1 Polyclonal Antibody, PeproTech®

MCP-1 Antibody (500-P34-1MG) in WB

Product Details

500-P34-1MG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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